Combinatorial cytotoxic effects of damnacanthal and doxorubicin against human breast cancer MCF-7 cells in vitro

Muhammad Yusran Abdul Aziz, Nadiah Abu, Swee Keong Yeap, Wan Yong Ho, Abdul Rahman Omar, Nor Hadiani Ismail, Syahida Ahmad, Mehdi R. Pirozyan, Nadeem M. Akhtar, Noorjahan Banu Alitheen

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Despite progressive research being done on drug therapy to treat breast cancer, the number of patients succumbing to the disease is still a major issue. Combinatorial treatment using different drugs and herbs to treat cancer patients is of major interest in scientists nowadays. Doxorubicin is one of the most used drugs to treat breast cancer patients. The combination of doxorubicin to other drugs such as tamoxifen has been reported. Nevertheless, the combination of doxorubicin with a natural product-derived agent has not been studied yet. Morinda citrifolia has always been sought out for its remarkable remedies. Damnacanthal, an anthraquinone that can be extracted from the roots of Morinda citrifolia is a promising compound that possesses a variety of biological properties. This study aimed to study the therapeutic effects of damnacanthal in combination with doxorubicin in breast cancer cells. Collectively, the combination of both these molecules enhanced the efficacy of induced cell death in MCF-7 as evidenced by the MTT assay, cell cycle, annexin V and expression of apoptosis-related genes and proteins. The effectiveness of doxorubicin as an anti-cancer drug was increased upon addition of damnacanthal. These results could provide a promising approach to treat breast cancer patients.

Original languageEnglish
Article number1228
JournalMolecules
Volume21
Issue number9
DOIs
Publication statusPublished - 1 Sep 2016

Fingerprint

MCF-7 Cells
breast
Doxorubicin
cancer
Breast Neoplasms
Morinda
drugs
cells
Pharmaceutical Preparations
Cells
Drug therapy
Anthraquinones
Annexin A5
Therapeutic Uses
Cell death
Tamoxifen
anthraquinones
Biological Products
apoptosis
chemotherapy

Keywords

  • Combination
  • Damnacanthal
  • Doxorubicin
  • MCF-7

ASJC Scopus subject areas

  • Organic Chemistry

Cite this

Aziz, M. Y. A., Abu, N., Yeap, S. K., Ho, W. Y., Omar, A. R., Ismail, N. H., ... Alitheen, N. B. (2016). Combinatorial cytotoxic effects of damnacanthal and doxorubicin against human breast cancer MCF-7 cells in vitro. Molecules, 21(9), [1228]. https://doi.org/10.3390/molecules21091228

Combinatorial cytotoxic effects of damnacanthal and doxorubicin against human breast cancer MCF-7 cells in vitro. / Aziz, Muhammad Yusran Abdul; Abu, Nadiah; Yeap, Swee Keong; Ho, Wan Yong; Omar, Abdul Rahman; Ismail, Nor Hadiani; Ahmad, Syahida; Pirozyan, Mehdi R.; Akhtar, Nadeem M.; Alitheen, Noorjahan Banu.

In: Molecules, Vol. 21, No. 9, 1228, 01.09.2016.

Research output: Contribution to journalArticle

Aziz, MYA, Abu, N, Yeap, SK, Ho, WY, Omar, AR, Ismail, NH, Ahmad, S, Pirozyan, MR, Akhtar, NM & Alitheen, NB 2016, 'Combinatorial cytotoxic effects of damnacanthal and doxorubicin against human breast cancer MCF-7 cells in vitro', Molecules, vol. 21, no. 9, 1228. https://doi.org/10.3390/molecules21091228
Aziz, Muhammad Yusran Abdul ; Abu, Nadiah ; Yeap, Swee Keong ; Ho, Wan Yong ; Omar, Abdul Rahman ; Ismail, Nor Hadiani ; Ahmad, Syahida ; Pirozyan, Mehdi R. ; Akhtar, Nadeem M. ; Alitheen, Noorjahan Banu. / Combinatorial cytotoxic effects of damnacanthal and doxorubicin against human breast cancer MCF-7 cells in vitro. In: Molecules. 2016 ; Vol. 21, No. 9.
@article{d422610705ca48478687780c4f3e0507,
title = "Combinatorial cytotoxic effects of damnacanthal and doxorubicin against human breast cancer MCF-7 cells in vitro",
abstract = "Despite progressive research being done on drug therapy to treat breast cancer, the number of patients succumbing to the disease is still a major issue. Combinatorial treatment using different drugs and herbs to treat cancer patients is of major interest in scientists nowadays. Doxorubicin is one of the most used drugs to treat breast cancer patients. The combination of doxorubicin to other drugs such as tamoxifen has been reported. Nevertheless, the combination of doxorubicin with a natural product-derived agent has not been studied yet. Morinda citrifolia has always been sought out for its remarkable remedies. Damnacanthal, an anthraquinone that can be extracted from the roots of Morinda citrifolia is a promising compound that possesses a variety of biological properties. This study aimed to study the therapeutic effects of damnacanthal in combination with doxorubicin in breast cancer cells. Collectively, the combination of both these molecules enhanced the efficacy of induced cell death in MCF-7 as evidenced by the MTT assay, cell cycle, annexin V and expression of apoptosis-related genes and proteins. The effectiveness of doxorubicin as an anti-cancer drug was increased upon addition of damnacanthal. These results could provide a promising approach to treat breast cancer patients.",
keywords = "Combination, Damnacanthal, Doxorubicin, MCF-7",
author = "Aziz, {Muhammad Yusran Abdul} and Nadiah Abu and Yeap, {Swee Keong} and Ho, {Wan Yong} and Omar, {Abdul Rahman} and Ismail, {Nor Hadiani} and Syahida Ahmad and Pirozyan, {Mehdi R.} and Akhtar, {Nadeem M.} and Alitheen, {Noorjahan Banu}",
year = "2016",
month = "9",
day = "1",
doi = "10.3390/molecules21091228",
language = "English",
volume = "21",
journal = "Molecules",
issn = "1420-3049",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "9",

}

TY - JOUR

T1 - Combinatorial cytotoxic effects of damnacanthal and doxorubicin against human breast cancer MCF-7 cells in vitro

AU - Aziz, Muhammad Yusran Abdul

AU - Abu, Nadiah

AU - Yeap, Swee Keong

AU - Ho, Wan Yong

AU - Omar, Abdul Rahman

AU - Ismail, Nor Hadiani

AU - Ahmad, Syahida

AU - Pirozyan, Mehdi R.

AU - Akhtar, Nadeem M.

AU - Alitheen, Noorjahan Banu

PY - 2016/9/1

Y1 - 2016/9/1

N2 - Despite progressive research being done on drug therapy to treat breast cancer, the number of patients succumbing to the disease is still a major issue. Combinatorial treatment using different drugs and herbs to treat cancer patients is of major interest in scientists nowadays. Doxorubicin is one of the most used drugs to treat breast cancer patients. The combination of doxorubicin to other drugs such as tamoxifen has been reported. Nevertheless, the combination of doxorubicin with a natural product-derived agent has not been studied yet. Morinda citrifolia has always been sought out for its remarkable remedies. Damnacanthal, an anthraquinone that can be extracted from the roots of Morinda citrifolia is a promising compound that possesses a variety of biological properties. This study aimed to study the therapeutic effects of damnacanthal in combination with doxorubicin in breast cancer cells. Collectively, the combination of both these molecules enhanced the efficacy of induced cell death in MCF-7 as evidenced by the MTT assay, cell cycle, annexin V and expression of apoptosis-related genes and proteins. The effectiveness of doxorubicin as an anti-cancer drug was increased upon addition of damnacanthal. These results could provide a promising approach to treat breast cancer patients.

AB - Despite progressive research being done on drug therapy to treat breast cancer, the number of patients succumbing to the disease is still a major issue. Combinatorial treatment using different drugs and herbs to treat cancer patients is of major interest in scientists nowadays. Doxorubicin is one of the most used drugs to treat breast cancer patients. The combination of doxorubicin to other drugs such as tamoxifen has been reported. Nevertheless, the combination of doxorubicin with a natural product-derived agent has not been studied yet. Morinda citrifolia has always been sought out for its remarkable remedies. Damnacanthal, an anthraquinone that can be extracted from the roots of Morinda citrifolia is a promising compound that possesses a variety of biological properties. This study aimed to study the therapeutic effects of damnacanthal in combination with doxorubicin in breast cancer cells. Collectively, the combination of both these molecules enhanced the efficacy of induced cell death in MCF-7 as evidenced by the MTT assay, cell cycle, annexin V and expression of apoptosis-related genes and proteins. The effectiveness of doxorubicin as an anti-cancer drug was increased upon addition of damnacanthal. These results could provide a promising approach to treat breast cancer patients.

KW - Combination

KW - Damnacanthal

KW - Doxorubicin

KW - MCF-7

UR - http://www.scopus.com/inward/record.url?scp=84987923868&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84987923868&partnerID=8YFLogxK

U2 - 10.3390/molecules21091228

DO - 10.3390/molecules21091228

M3 - Article

VL - 21

JO - Molecules

JF - Molecules

SN - 1420-3049

IS - 9

M1 - 1228

ER -