Combination of zerumbone and cisplatin to treat cervical intraepithelial neoplasia in female BALB/c mice

Ahmad Bustamam Abdul, Siddig Ibrahim Abdelwahab, Johari Jalinas, Adel Sharaf Al-Zubairi, Manal Mohamed Elhassan Taha

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Recent in vitro and in vivo studies have demonstrated that zerumbone (ZER) possesses anticancer properties. The main objective of this study was to examine the effectiveness of the combination of ZER and cisplatin (CIS) to treat cervical intraepithelial neoplasia (CIN) in vivo. Microculture tetrazolium assay and immunohistochemistry of proliferating cellular nuclear antigen were used to study the antitumor effect of ZER.Prenatally exposed female BALB/c micewere used as a model. The progenies with CIN were injected peritoneally with isotonic sodium chloride solution (positive control), CIS, ZER,and a combination of both compounds. All treated and untreated mice were humanely killed, and serum and cervix were obtained for interleukin 6 analysis and histopathologic studies using hematoxylin and eosin staining, respectively. Zerumbone has revealed an antitumor effect on human cervical cancer cells and downregulates immunoexpression of proliferating cellular nuclear antigen (P < 0.05). In vivo study indicates that ZER at 16 mg/kg and CIS at 10 mg/kg have a regressing effect on CIN. The combination of ZER and CIS also showed similar effectiveness in regressing CIN. Our results indicate that the combination of ZER and CIS has modulated the serum level of interleukin 6 when compared with that in mice treated with isotonic sodium chloride solution (P < 0.05). The effectiveness of combining ZER and CIS could be further explored as a new therapeutic intervention of early precancerous stages of carcinogenesis before the invasive stage begins.

Original languageEnglish
Pages (from-to)1004-1010
Number of pages7
JournalInternational Journal of Gynecological Cancer
Volume19
Issue number6
DOIs
Publication statusPublished - Aug 2009
Externally publishedYes

Fingerprint

Cervical Intraepithelial Neoplasia
Cisplatin
Nuclear Antigens
Sodium Chloride
Interleukin-6
zerumbone
Hematoxylin
Eosine Yellowish-(YS)
Serum
Cervix Uteri
Uterine Cervical Neoplasms
Carcinogenesis
Down-Regulation
Immunohistochemistry
Staining and Labeling

Keywords

  • Cervical intraepithelial neoplasia
  • Cisplatin
  • Synergy
  • Zerumbone

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Oncology

Cite this

Combination of zerumbone and cisplatin to treat cervical intraepithelial neoplasia in female BALB/c mice. / Abdul, Ahmad Bustamam; Abdelwahab, Siddig Ibrahim; Jalinas, Johari; Al-Zubairi, Adel Sharaf; Taha, Manal Mohamed Elhassan.

In: International Journal of Gynecological Cancer, Vol. 19, No. 6, 08.2009, p. 1004-1010.

Research output: Contribution to journalArticle

Abdul, Ahmad Bustamam ; Abdelwahab, Siddig Ibrahim ; Jalinas, Johari ; Al-Zubairi, Adel Sharaf ; Taha, Manal Mohamed Elhassan. / Combination of zerumbone and cisplatin to treat cervical intraepithelial neoplasia in female BALB/c mice. In: International Journal of Gynecological Cancer. 2009 ; Vol. 19, No. 6. pp. 1004-1010.
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