Co-solvent selection for supercritical fluid extraction of essential oil and bioactive compounds from polygonum minus

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Abstract

This study evaluated the biological activity (antioxidant assay) of Polygonum minusextracted using Supercritical Fluid Extraction (SFE) added with different types of co-solvents. The seven co-solvents employed were water, methanol, ethanol, 50% methanol, 50% ethanol, 70% methanol and 70% ethanol for selection of the best co-solvent prior to optimization of SFE. 70% methanol produced the highest total yield of extract (33.1%) compared to other co-solvents. The antioxidant capacity was then evaluated using four different assays: the total phenolic content (TP), the total flavonoid content(TF), the ferric reducing/antioxidant power (FRAP) and the free radical-scavenging capacity of 2,2-diphenyl-1-picrylhydrazyl (DPPH). The highest TP and TF were from 70% methanol extract (11.2 ± 0.15 mg GAE/g sample(mg GAE/g) and 11.9 ± 0.03 mg CAE/g sample (mg CEQ/g) respectively). 70% metanolextract also showed the highest FRAP value (346.7 ± 0.66 μmol Fe (II)/g sample) and the highest percentage of DPPH radical inhibition was also shown by 70% methanol extract (88.7 ± 0.40%). There was a positive correlation between the antioxidant capacity (FRAP and DPPH) with those of TP and TF contents. Therefore, the best co-solvent chosen for further optimization of SFE is 70% methanol.

Original languageEnglish
Pages (from-to)19-26
Number of pages8
JournalASEAN Journal of Chemical Engineering
Volume12
Issue number2
Publication statusPublished - 2012

Fingerprint

Essential oils
Supercritical fluids
Volatile Oils
Methanol
Antioxidants
Flavonoids
Ethanol
Assays
Computer aided engineering
Scavenging
Bioactivity
Free radicals
Free Radicals
Water

Keywords

  • Antioxidant capacity
  • Biological activity
  • Co-solvent
  • Pheliccontent
  • Polygonum minus
  • Supercritical fluid extraction

ASJC Scopus subject areas

  • Chemical Engineering(all)

Cite this

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title = "Co-solvent selection for supercritical fluid extraction of essential oil and bioactive compounds from polygonum minus",
abstract = "This study evaluated the biological activity (antioxidant assay) of Polygonum minusextracted using Supercritical Fluid Extraction (SFE) added with different types of co-solvents. The seven co-solvents employed were water, methanol, ethanol, 50{\%} methanol, 50{\%} ethanol, 70{\%} methanol and 70{\%} ethanol for selection of the best co-solvent prior to optimization of SFE. 70{\%} methanol produced the highest total yield of extract (33.1{\%}) compared to other co-solvents. The antioxidant capacity was then evaluated using four different assays: the total phenolic content (TP), the total flavonoid content(TF), the ferric reducing/antioxidant power (FRAP) and the free radical-scavenging capacity of 2,2-diphenyl-1-picrylhydrazyl (DPPH). The highest TP and TF were from 70{\%} methanol extract (11.2 ± 0.15 mg GAE/g sample(mg GAE/g) and 11.9 ± 0.03 mg CAE/g sample (mg CEQ/g) respectively). 70{\%} metanolextract also showed the highest FRAP value (346.7 ± 0.66 μmol Fe (II)/g sample) and the highest percentage of DPPH radical inhibition was also shown by 70{\%} methanol extract (88.7 ± 0.40{\%}). There was a positive correlation between the antioxidant capacity (FRAP and DPPH) with those of TP and TF contents. Therefore, the best co-solvent chosen for further optimization of SFE is 70{\%} methanol.",
keywords = "Antioxidant capacity, Biological activity, Co-solvent, Pheliccontent, Polygonum minus, Supercritical fluid extraction",
author = "Masturah Markom and Norsyamimi Hassim and Nurina Anuar and Baharum, {Syarul Nataqain}",
year = "2012",
language = "English",
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AU - Baharum, Syarul Nataqain

PY - 2012

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N2 - This study evaluated the biological activity (antioxidant assay) of Polygonum minusextracted using Supercritical Fluid Extraction (SFE) added with different types of co-solvents. The seven co-solvents employed were water, methanol, ethanol, 50% methanol, 50% ethanol, 70% methanol and 70% ethanol for selection of the best co-solvent prior to optimization of SFE. 70% methanol produced the highest total yield of extract (33.1%) compared to other co-solvents. The antioxidant capacity was then evaluated using four different assays: the total phenolic content (TP), the total flavonoid content(TF), the ferric reducing/antioxidant power (FRAP) and the free radical-scavenging capacity of 2,2-diphenyl-1-picrylhydrazyl (DPPH). The highest TP and TF were from 70% methanol extract (11.2 ± 0.15 mg GAE/g sample(mg GAE/g) and 11.9 ± 0.03 mg CAE/g sample (mg CEQ/g) respectively). 70% metanolextract also showed the highest FRAP value (346.7 ± 0.66 μmol Fe (II)/g sample) and the highest percentage of DPPH radical inhibition was also shown by 70% methanol extract (88.7 ± 0.40%). There was a positive correlation between the antioxidant capacity (FRAP and DPPH) with those of TP and TF contents. Therefore, the best co-solvent chosen for further optimization of SFE is 70% methanol.

AB - This study evaluated the biological activity (antioxidant assay) of Polygonum minusextracted using Supercritical Fluid Extraction (SFE) added with different types of co-solvents. The seven co-solvents employed were water, methanol, ethanol, 50% methanol, 50% ethanol, 70% methanol and 70% ethanol for selection of the best co-solvent prior to optimization of SFE. 70% methanol produced the highest total yield of extract (33.1%) compared to other co-solvents. The antioxidant capacity was then evaluated using four different assays: the total phenolic content (TP), the total flavonoid content(TF), the ferric reducing/antioxidant power (FRAP) and the free radical-scavenging capacity of 2,2-diphenyl-1-picrylhydrazyl (DPPH). The highest TP and TF were from 70% methanol extract (11.2 ± 0.15 mg GAE/g sample(mg GAE/g) and 11.9 ± 0.03 mg CAE/g sample (mg CEQ/g) respectively). 70% metanolextract also showed the highest FRAP value (346.7 ± 0.66 μmol Fe (II)/g sample) and the highest percentage of DPPH radical inhibition was also shown by 70% methanol extract (88.7 ± 0.40%). There was a positive correlation between the antioxidant capacity (FRAP and DPPH) with those of TP and TF contents. Therefore, the best co-solvent chosen for further optimization of SFE is 70% methanol.

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