Co-solvent selection for supercritical fluid extraction of astaxanthin and other carotenoids from Penaeus monodon waste

Shazana Azfar Radzali, Badlishah Sham Baharin, Rashidi Othman, Masturah Markom, Russly Abdul Rahman

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

In recent years, astaxanthin is claimed to have a 10 times higher antioxidant activity than that of other carotenoids such as lutein, zeaxanthin, canthaxanthin, and β-carotene; the antioxidant activity of astaxanthin is 100 times higher than that of α-tocopherol. Penaeus monodon (tiger shrimp) is the largest commercially available shrimp species and its waste is a rich source of carotenoids such as astaxanthin and its esters. The efficient and environment-friendly recovery of astaxanthins was accomplished by using a supercritical fluid extraction (SFE) technique. The effects of different co-solvents and their concentrations on the yield and composition of the extract were investigated. The following co-solvents were studied prior to the optimization of the SFE technique: ethanol, water, methanol, 50% (v/v) ethanol in water, 50% (v/v) methanol in water, 70% (v/v) ethanol in water, and 70% (v/v) methanol in water. The ethanol extract produced the highest carotenoid yield (84.02 ± 0.8 μg/g) dry weight (DW) with 97.1% recovery. The ethanol extract also produced the highest amount of the extracted astaxanthin complex (58.03 ± 0.1 μg/g DW) and the free astaxanthin content (12.25 ± 0.9 μg/g DW) in the extract. Lutein and β-carotene were the other carotenoids identified. Therefore, ethanol was chosen for further optimization studies.

Original languageEnglish
Pages (from-to)769-777
Number of pages9
JournalJournal of Oleo Science
Volume63
Issue number8
DOIs
Publication statusPublished - 2014

Fingerprint

Penaeidae
Supercritical Fluid Chromatography
Supercritical fluids
Carotenoids
Ethanol
Water
Methanol
Lutein
Antioxidants
Weights and Measures
Canthaxanthin
Tigers
Recovery
Tocopherols
astaxanthine
Esters
Chemical analysis

Keywords

  • Astaxanthin
  • Carotenoids
  • HPLC
  • Penaeus monodon
  • SFE

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Chemistry(all)
  • Medicine(all)

Cite this

Co-solvent selection for supercritical fluid extraction of astaxanthin and other carotenoids from Penaeus monodon waste. / Radzali, Shazana Azfar; Baharin, Badlishah Sham; Othman, Rashidi; Markom, Masturah; Rahman, Russly Abdul.

In: Journal of Oleo Science, Vol. 63, No. 8, 2014, p. 769-777.

Research output: Contribution to journalArticle

Radzali, Shazana Azfar ; Baharin, Badlishah Sham ; Othman, Rashidi ; Markom, Masturah ; Rahman, Russly Abdul. / Co-solvent selection for supercritical fluid extraction of astaxanthin and other carotenoids from Penaeus monodon waste. In: Journal of Oleo Science. 2014 ; Vol. 63, No. 8. pp. 769-777.
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