Cloning, purification, crystallization and preliminary X-ray analysis of the Burkholderia pseudomallei L1 ribosomal protein

Abd Ghani Abd Aziz, Sergey N. Ruzheinikov, Svetlana E. Sedelnikova, Rahmah Mohamed, Sheila Nathan, Patrick J. Baker, David W. Rice

Research output: Contribution to journalArticle

Abstract

The gene encoding the L1 ribosomal protein from Burkholderia pseudomallei strain D286 has been cloned into the pETBLUE-1 vector system, overexpressed in Escherichia coli and purified. Crystals of the native protein were grown by the hanging-drop vapour-diffusion technique using PEG 3350 as a precipitant and diffracted to beyond 1.65 Å resolution. The crystals belonged to space group P2 12 12, with unit-cell parameters a = 53.6, b = 127.1, c = 31.8 Å and with a single molecule in the asymmetric unit.

Original languageEnglish
Pages (from-to)347-350
Number of pages4
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume68
Issue number3
DOIs
Publication statusPublished - Mar 2012

Fingerprint

Burkholderia pseudomallei
Cloning
X ray analysis
Crystallization
purification
Purification
Organism Cloning
X-Rays
crystallization
Escherichia coli
proteins
Crystals
Gene encoding
Escherichia
genes
Genes
crystals
coding
Proteins
x rays

Keywords

  • BPSL3224
  • Burkholderia pseudomallei
  • L1 ribosomal proteins

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Structural Biology
  • Genetics
  • Condensed Matter Physics

Cite this

Cloning, purification, crystallization and preliminary X-ray analysis of the Burkholderia pseudomallei L1 ribosomal protein. / Abd Aziz, Abd Ghani; Ruzheinikov, Sergey N.; Sedelnikova, Svetlana E.; Mohamed, Rahmah; Nathan, Sheila; Baker, Patrick J.; Rice, David W.

In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 68, No. 3, 03.2012, p. 347-350.

Research output: Contribution to journalArticle

Abd Aziz, Abd Ghani ; Ruzheinikov, Sergey N. ; Sedelnikova, Svetlana E. ; Mohamed, Rahmah ; Nathan, Sheila ; Baker, Patrick J. ; Rice, David W. / Cloning, purification, crystallization and preliminary X-ray analysis of the Burkholderia pseudomallei L1 ribosomal protein. In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications. 2012 ; Vol. 68, No. 3. pp. 347-350.
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