Cloning, purification and crystallographic analysis of a hypothetical protein, BPSL1549, from Burkholderia pseudomallei

Abimael Cruz-Migoni, Sergey N. Ruzheinikov, Svetlana E. Sedelnikova, Barbara Obeng, Sylvia Chieng, Rahmah Mohamed, Sheila Nathan, Patrick J. Baker, David W. Rice

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Burkholderia pseudomallei BPSL1549, a putative protein of unknown function, has been overexpressed in Escherichia coli, purified and subsequently crystallized by the hanging-drop vapour-diffusion method using PEG as a precipitant to give crystals with overall dimensions of 0.15 × 0.15 × 0.1 mm. Native data were collected to 1.47 Å resolution at the European Synchrotron Radiation Facility (ESRF). The crystals belonged to space group P2 12 12 1, with unit-cell parameters a = 37.1, b = 45.4, c = 111.9 Å and with a single polypeptide chain in the asymmetric unit.

Original languageEnglish
Pages (from-to)1623-1626
Number of pages4
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume67
Issue number12
DOIs
Publication statusPublished - Dec 2011

Fingerprint

Burkholderia pseudomallei
Synchrotrons
Cloning
purification
Purification
Organism Cloning
Radiation
Escherichia coli
proteins
Peptides
Crystals
polypeptides
Escherichia
Synchrotron radiation
Polyethylene glycols
crystals
synchrotron radiation
Proteins
Vapors
vapors

Keywords

  • BPSL1549
  • Burkholderia pseudomallei
  • pathogens

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Structural Biology
  • Genetics
  • Condensed Matter Physics

Cite this

Cloning, purification and crystallographic analysis of a hypothetical protein, BPSL1549, from Burkholderia pseudomallei. / Cruz-Migoni, Abimael; Ruzheinikov, Sergey N.; Sedelnikova, Svetlana E.; Obeng, Barbara; Chieng, Sylvia; Mohamed, Rahmah; Nathan, Sheila; Baker, Patrick J.; Rice, David W.

In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 67, No. 12, 12.2011, p. 1623-1626.

Research output: Contribution to journalArticle

Cruz-Migoni, Abimael ; Ruzheinikov, Sergey N. ; Sedelnikova, Svetlana E. ; Obeng, Barbara ; Chieng, Sylvia ; Mohamed, Rahmah ; Nathan, Sheila ; Baker, Patrick J. ; Rice, David W. / Cloning, purification and crystallographic analysis of a hypothetical protein, BPSL1549, from Burkholderia pseudomallei. In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications. 2011 ; Vol. 67, No. 12. pp. 1623-1626.
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