Cloning of high activity xylanase gene from Bacillus pumilus PJ19

A. Hamzah, N. Abdulrashid

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    The xylanase gene from Bacillus pumilus PJ19 amplified by polymerase chain reaction (PCR) was cloned into pCR™ II vector and transformed into Escherichia coli strain INVαF′. Starting from an ATG as an initiator codon, an open reading frame coding for 202 amino acids was obtained. The recombinant xylanase sequence showed a 96% homology with the xylanase sequence from B. pumilus IPO strain and had an estimated molecular weight of 22,474. Xylanase activity expressed by E. coli INVαF′ harboring the cloned gene was located primarily in the cytoplasmic fraction.

    Original languageEnglish
    Pages (from-to)365-369
    Number of pages5
    JournalJournal of Biochemistry, Molecular Biology and Biophysics
    Volume6
    Issue number5
    DOIs
    Publication statusPublished - Oct 2002

    Fingerprint

    Cloning
    Bacilli
    Escherichia coli
    Organism Cloning
    Genes
    Endo-1,4-beta Xylanases
    Initiator Codon
    Polymerase chain reaction
    Open Reading Frames
    Molecular Weight
    Molecular weight
    Amino Acids
    Polymerase Chain Reaction
    Bacillus pumilus

    Keywords

    • Bacillus pumilus PJ19
    • Nucleotide sequence
    • PCR cloning
    • Xylanase

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology
    • Genetics
    • Biophysics

    Cite this

    Cloning of high activity xylanase gene from Bacillus pumilus PJ19. / Hamzah, A.; Abdulrashid, N.

    In: Journal of Biochemistry, Molecular Biology and Biophysics, Vol. 6, No. 5, 10.2002, p. 365-369.

    Research output: Contribution to journalArticle

    @article{4b5541a82afc49b9a90d292d40e0039a,
    title = "Cloning of high activity xylanase gene from Bacillus pumilus PJ19",
    abstract = "The xylanase gene from Bacillus pumilus PJ19 amplified by polymerase chain reaction (PCR) was cloned into pCR™ II vector and transformed into Escherichia coli strain INVαF′. Starting from an ATG as an initiator codon, an open reading frame coding for 202 amino acids was obtained. The recombinant xylanase sequence showed a 96{\%} homology with the xylanase sequence from B. pumilus IPO strain and had an estimated molecular weight of 22,474. Xylanase activity expressed by E. coli INVαF′ harboring the cloned gene was located primarily in the cytoplasmic fraction.",
    keywords = "Bacillus pumilus PJ19, Nucleotide sequence, PCR cloning, Xylanase",
    author = "A. Hamzah and N. Abdulrashid",
    year = "2002",
    month = "10",
    doi = "10.1080/1025814021000024307",
    language = "English",
    volume = "6",
    pages = "365--369",
    journal = "Journal of Biochemistry, Molecular Biology and Biophysics",
    issn = "1025-8140",
    publisher = "Taylor and Francis Ltd.",
    number = "5",

    }

    TY - JOUR

    T1 - Cloning of high activity xylanase gene from Bacillus pumilus PJ19

    AU - Hamzah, A.

    AU - Abdulrashid, N.

    PY - 2002/10

    Y1 - 2002/10

    N2 - The xylanase gene from Bacillus pumilus PJ19 amplified by polymerase chain reaction (PCR) was cloned into pCR™ II vector and transformed into Escherichia coli strain INVαF′. Starting from an ATG as an initiator codon, an open reading frame coding for 202 amino acids was obtained. The recombinant xylanase sequence showed a 96% homology with the xylanase sequence from B. pumilus IPO strain and had an estimated molecular weight of 22,474. Xylanase activity expressed by E. coli INVαF′ harboring the cloned gene was located primarily in the cytoplasmic fraction.

    AB - The xylanase gene from Bacillus pumilus PJ19 amplified by polymerase chain reaction (PCR) was cloned into pCR™ II vector and transformed into Escherichia coli strain INVαF′. Starting from an ATG as an initiator codon, an open reading frame coding for 202 amino acids was obtained. The recombinant xylanase sequence showed a 96% homology with the xylanase sequence from B. pumilus IPO strain and had an estimated molecular weight of 22,474. Xylanase activity expressed by E. coli INVαF′ harboring the cloned gene was located primarily in the cytoplasmic fraction.

    KW - Bacillus pumilus PJ19

    KW - Nucleotide sequence

    KW - PCR cloning

    KW - Xylanase

    UR - http://www.scopus.com/inward/record.url?scp=0036805148&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0036805148&partnerID=8YFLogxK

    U2 - 10.1080/1025814021000024307

    DO - 10.1080/1025814021000024307

    M3 - Article

    VL - 6

    SP - 365

    EP - 369

    JO - Journal of Biochemistry, Molecular Biology and Biophysics

    JF - Journal of Biochemistry, Molecular Biology and Biophysics

    SN - 1025-8140

    IS - 5

    ER -