Cloning, expression and display of the PreS domain of hepatitis B virus on filamentous bacteriophage M13

W. L. Kok, K. Yusoff, Sheila Nathan, W. S. Tan

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The PreS domain of hepatitis B virus (HBV) is believed to be involved in virion assembly and attachment to a hepatocyte receptor during infection. In order to study the functions of this region, we fused it to the g3p protein of bacteriophage M13 that allows the fusion protein to be displayed at the tip of the filament. The fusion protein was detected by the anti-E tag antibody on a Western blot. The polypeptide in a soluble form was produced by transfecting a non-suppressor E. coli host cell with the recombinant phagemid. The soluble protein was detected in cytoplasm, in the periplasmic space and also in the medium. The functional display of the PreS domain would provide an alternative means to study its interactions with the nucleocapsid and hepatocytes.

Original languageEnglish
Pages (from-to)55-58
Number of pages4
JournalJournal of Biochemistry, Molecular Biology and Biophysics
Volume6
Issue number1
DOIs
Publication statusPublished - Feb 2002
Externally publishedYes

Fingerprint

Inovirus
Bacteriophage M13
Bacteriophages
Cloning
Viruses
Hepatitis B virus
Organism Cloning
Display devices
Hepatocytes
Proteins
Fusion reactions
Nucleocapsid
Periplasm
Virion
Escherichia coli
Cytoplasm
Western Blotting
Peptides
Antibodies
Infection

Keywords

  • Fusion protein
  • Hepatitis B virus
  • Phage display
  • Surface antigen

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics
  • Biophysics

Cite this

Cloning, expression and display of the PreS domain of hepatitis B virus on filamentous bacteriophage M13. / Kok, W. L.; Yusoff, K.; Nathan, Sheila; Tan, W. S.

In: Journal of Biochemistry, Molecular Biology and Biophysics, Vol. 6, No. 1, 02.2002, p. 55-58.

Research output: Contribution to journalArticle

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