Cloning and expression of N -glycosylation-related mannosidase from Glaciozyma antarctica for the production of a mannosynthase

Dharshini Elangovan, Shazilah Kamaruddin, Noor Haza Fazlin Hashim, Farah Diba Abu Bakar, Abdul Munir Abd. Murad, Nor Muhammad Mahadi, Sarah Ann Allman, Muhammad Mukram Mohamed Mackeen

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The controlled synthesis of oligosaccharides is of growing interest due to the important roles of oligosaccharides in various biological processes. Enzymatic synthesis enables regio- and stereo-selective control during synthesis which still remains a challenge using total chemical synthesis. In this study, endoplasmic reticulum 1,2-α-mannosidase from Glaciozyma antractica was recombinantly expressed in Pichia pastoris. The gene sequence for ER mannosidase was obtained from the Glaciozyma antractica database. The BLAST (Basic Local Alignment Search Tool) results from bioinformatics screening showed that ER mannosidase had 41 % identity with the equivalent mannosidases from Sacchromyces cerevesiae. ER mannosidase from G. antartica was then cloned into the pPICZαC expression vector and used to transform in the host Pichia pastoris X33 cells. The ER mannosidase (MW∼58 kDa) was successfully expressed at 25°C with 1.0 % methanol induction.

Original languageEnglish
Title of host publication2016 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2016 Postgraduate Colloquium
PublisherAmerican Institute of Physics Inc.
Volume1784
ISBN (Electronic)9780735414464
DOIs
Publication statusPublished - 17 Nov 2016
Event2016 Postgraduate Colloquium of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology, UKM FST 2016 - Selangor, Malaysia
Duration: 13 Apr 201614 Apr 2016

Other

Other2016 Postgraduate Colloquium of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology, UKM FST 2016
CountryMalaysia
CitySelangor
Period13/4/1614/4/16

Fingerprint

Antarctic regions
synthesis
endoplasmic reticulum
genes
induction
screening
methyl alcohol
alignment
cells

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Elangovan, D., Kamaruddin, S., Hashim, N. H. F., Abu Bakar, F. D., Abd. Murad, A. M., Mahadi, N. M., ... Mohamed Mackeen, M. M. (2016). Cloning and expression of N -glycosylation-related mannosidase from Glaciozyma antarctica for the production of a mannosynthase. In 2016 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2016 Postgraduate Colloquium (Vol. 1784). [030011] American Institute of Physics Inc.. https://doi.org/10.1063/1.4966749

Cloning and expression of N -glycosylation-related mannosidase from Glaciozyma antarctica for the production of a mannosynthase. / Elangovan, Dharshini; Kamaruddin, Shazilah; Hashim, Noor Haza Fazlin; Abu Bakar, Farah Diba; Abd. Murad, Abdul Munir; Mahadi, Nor Muhammad; Allman, Sarah Ann; Mohamed Mackeen, Muhammad Mukram.

2016 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2016 Postgraduate Colloquium. Vol. 1784 American Institute of Physics Inc., 2016. 030011.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Elangovan, D, Kamaruddin, S, Hashim, NHF, Abu Bakar, FD, Abd. Murad, AM, Mahadi, NM, Allman, SA & Mohamed Mackeen, MM 2016, Cloning and expression of N -glycosylation-related mannosidase from Glaciozyma antarctica for the production of a mannosynthase. in 2016 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2016 Postgraduate Colloquium. vol. 1784, 030011, American Institute of Physics Inc., 2016 Postgraduate Colloquium of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology, UKM FST 2016, Selangor, Malaysia, 13/4/16. https://doi.org/10.1063/1.4966749
Elangovan D, Kamaruddin S, Hashim NHF, Abu Bakar FD, Abd. Murad AM, Mahadi NM et al. Cloning and expression of N -glycosylation-related mannosidase from Glaciozyma antarctica for the production of a mannosynthase. In 2016 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2016 Postgraduate Colloquium. Vol. 1784. American Institute of Physics Inc. 2016. 030011 https://doi.org/10.1063/1.4966749
Elangovan, Dharshini ; Kamaruddin, Shazilah ; Hashim, Noor Haza Fazlin ; Abu Bakar, Farah Diba ; Abd. Murad, Abdul Munir ; Mahadi, Nor Muhammad ; Allman, Sarah Ann ; Mohamed Mackeen, Muhammad Mukram. / Cloning and expression of N -glycosylation-related mannosidase from Glaciozyma antarctica for the production of a mannosynthase. 2016 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2016 Postgraduate Colloquium. Vol. 1784 American Institute of Physics Inc., 2016.
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AU - Abd. Murad, Abdul Munir

AU - Mahadi, Nor Muhammad

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