Cloning and expression of N -glycosylation-related glucosidase from Glaciozyma antarctica

Noor Liana Mat Yajit, Shazilah Kamaruddin, Noor Haza Fazlin Hashim, Farah Diba Abu Bakar, Abdul Munir Abd. Murad, Nor Muhammad Mahadi, Muhammad Mukram Mohamed Mackeen

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The need for functional oligosaccharides in various field is ever growing. The enzymatic approach for synthesis of oligosaccharides is advantageous over traditional chemical synthesis because of the regio- and stereo- selectivity that can be achieved without the need for protection chemistry. In this study, the α-glucosidase I protein sequence from Saccharomyces cerevisiae (UniProt database) was compared using Basic Local Alignment Search Tool (BLAST) with Glaciozyma antarctica genome database. Results showed 33% identity and an E-value of 1 × 10-125 for α-glucosidase I. The gene was amplified, cloned into the pPICZα C vector and used to transform Pichia pastoris X-33 cells. Soluble expression of α-Glucosidase I (∼91 kDa) was achieved at 28°C with 1.0 % of methanol.

Original languageEnglish
Title of host publication2016 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2016 Postgraduate Colloquium
PublisherAmerican Institute of Physics Inc.
Volume1784
ISBN (Electronic)9780735414464
DOIs
Publication statusPublished - 17 Nov 2016
Event2016 Postgraduate Colloquium of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology, UKM FST 2016 - Selangor, Malaysia
Duration: 13 Apr 201614 Apr 2016

Other

Other2016 Postgraduate Colloquium of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology, UKM FST 2016
CountryMalaysia
CitySelangor
Period13/4/1614/4/16

Fingerprint

Antarctic regions
saccharomyces
genome
synthesis
genes
methyl alcohol
selectivity
alignment
chemistry
proteins
cells

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Yajit, N. L. M., Kamaruddin, S., Hashim, N. H. F., Abu Bakar, F. D., Abd. Murad, A. M., Mahadi, N. M., & Mohamed Mackeen, M. M. (2016). Cloning and expression of N -glycosylation-related glucosidase from Glaciozyma antarctica. In 2016 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2016 Postgraduate Colloquium (Vol. 1784). [020004] American Institute of Physics Inc.. https://doi.org/10.1063/1.4966714

Cloning and expression of N -glycosylation-related glucosidase from Glaciozyma antarctica. / Yajit, Noor Liana Mat; Kamaruddin, Shazilah; Hashim, Noor Haza Fazlin; Abu Bakar, Farah Diba; Abd. Murad, Abdul Munir; Mahadi, Nor Muhammad; Mohamed Mackeen, Muhammad Mukram.

2016 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2016 Postgraduate Colloquium. Vol. 1784 American Institute of Physics Inc., 2016. 020004.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Yajit, NLM, Kamaruddin, S, Hashim, NHF, Abu Bakar, FD, Abd. Murad, AM, Mahadi, NM & Mohamed Mackeen, MM 2016, Cloning and expression of N -glycosylation-related glucosidase from Glaciozyma antarctica. in 2016 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2016 Postgraduate Colloquium. vol. 1784, 020004, American Institute of Physics Inc., 2016 Postgraduate Colloquium of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology, UKM FST 2016, Selangor, Malaysia, 13/4/16. https://doi.org/10.1063/1.4966714
Yajit NLM, Kamaruddin S, Hashim NHF, Abu Bakar FD, Abd. Murad AM, Mahadi NM et al. Cloning and expression of N -glycosylation-related glucosidase from Glaciozyma antarctica. In 2016 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2016 Postgraduate Colloquium. Vol. 1784. American Institute of Physics Inc. 2016. 020004 https://doi.org/10.1063/1.4966714
Yajit, Noor Liana Mat ; Kamaruddin, Shazilah ; Hashim, Noor Haza Fazlin ; Abu Bakar, Farah Diba ; Abd. Murad, Abdul Munir ; Mahadi, Nor Muhammad ; Mohamed Mackeen, Muhammad Mukram. / Cloning and expression of N -glycosylation-related glucosidase from Glaciozyma antarctica. 2016 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2016 Postgraduate Colloquium. Vol. 1784 American Institute of Physics Inc., 2016.
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