Cloning and characterisation of multiple ferritin isoforms in the Atlantic salmon (Salmo salar)

Jun Hoe Lee, Nicholas J. Pooley, Adura Mohd-Adnan, Samuel A M Martin

    Research output: Contribution to journalArticle

    11 Citations (Scopus)

    Abstract

    Ferritin is a highly-conserved iron-storage protein that has also been identified as an acute phase protein within the innate immune system. The iron-storage function is mediated through complementary roles played by heavy (H)-chain subunit as well as the light (L) in mammals or middle (M)-chain in teleosts, respectively. In this study, we report the identification of five ferritin subunits (H1, H2, M1, M2, M3) in the Atlantic salmon that were supported by the presence of iron-regulatory regions, gene structure, conserved domains and phylogenetic analysis. Tissue distribution analysis across eight different tissues showed that each of these isoforms is differentially expressed. We also examined the expression of the ferritin isoforms in the liver and kidney of juvenile Atlantic salmon that was challenged with Aeromonas salmonicida as well as in muscle cell culture stimulated with interleukin-1β. We found that each isoform displayed unique expression profiles, and in certain conditions the expressions between the isoforms were completely diametrical to each other. Our study is the first report of multiple ferritin isoforms from both the H- and M-chains in a vertebrate species, as well as ferritin isoforms that showed decreased expression in response to infection. Taken together, the results of our study suggest the possibility of functional differences between the H- and M-chain isoforms in terms of tissue localisation, transcriptional response to bacterial exposure and stimulation by specific immune factors.

    Original languageEnglish
    Article numbere103729
    JournalPLoS One
    Volume9
    Issue number7
    DOIs
    Publication statusPublished - 31 Jul 2014

    Fingerprint

    Salmo salar
    Cloning
    ferritin
    Ferritins
    Organism Cloning
    molecular cloning
    Protein Isoforms
    iron
    Iron
    Tissue
    Aeromonas salmonicida
    acute phase proteins
    interleukin-1
    tissue distribution
    storage proteins
    myocytes
    Mammals
    Acute-Phase Proteins
    Immune system
    Nucleic Acid Regulatory Sequences

    ASJC Scopus subject areas

    • Agricultural and Biological Sciences(all)
    • Biochemistry, Genetics and Molecular Biology(all)
    • Medicine(all)

    Cite this

    Cloning and characterisation of multiple ferritin isoforms in the Atlantic salmon (Salmo salar). / Lee, Jun Hoe; Pooley, Nicholas J.; Mohd-Adnan, Adura; Martin, Samuel A M.

    In: PLoS One, Vol. 9, No. 7, e103729, 31.07.2014.

    Research output: Contribution to journalArticle

    Lee, Jun Hoe ; Pooley, Nicholas J. ; Mohd-Adnan, Adura ; Martin, Samuel A M. / Cloning and characterisation of multiple ferritin isoforms in the Atlantic salmon (Salmo salar). In: PLoS One. 2014 ; Vol. 9, No. 7.
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