Pengklonan dan pencirian klon CUKMCS yang mengekodkan kapsaisin sintase (Cs)

Translated title of the contribution: Cloning and characterisation of CUKMCS clone encoding capsaicin synthase

Research output: Contribution to journalArticle

Abstract

Capsaicinoid, the main alkaloid compound that gives pungent taste to peppers is restricted to the genus of Capsicum. It consists of two major components which are capsaicin and dihydrocapsaicin. Here we reported the isolation o/capsaicin synthase (Cs) cDNA via RT-PCR method and the resulting cloned was named as CUKMCS with the expected size of 981 bp. Homology search via the blastx and blastp program from the NCBI database sliowed that CUKMCS lias a high homology to the Cs in Capsicum frutescens, Capsicum annuum and Capsicum chacoense. The predicted size of CUKMCS protein is around 36 kDa. Transcripts expression in 5 different tissues revealed that Cs transcripts were detected in the placental tissue, mesocarp and seed while no corresponding expression was detected in the leaf and root.

Original languageUndefined/Unknown
Pages (from-to)129-134
Number of pages6
JournalSains Malaysiana
Volume39
Issue number1
Publication statusPublished - Feb 2010

Fingerprint

capsaicin
molecular cloning
clones
Capsicum chacoense
Capsicum frutescens
mesocarp
Capsicum
sweet peppers
Capsicum annuum
alkaloids
reverse transcriptase polymerase chain reaction
seeds
leaves
proteins
tissues
methodology

Keywords

  • Capsaicin synthase Cs
  • Capsaicinoid
  • Capsicum frutescens
  • mRNA transcript

ASJC Scopus subject areas

  • General

Cite this

Pengklonan dan pencirian klon CUKMCS yang mengekodkan kapsaisin sintase (Cs). / Md Isa, Nurulhikma; Ismail, Ismanizan; Zainal, Zamri.

In: Sains Malaysiana, Vol. 39, No. 1, 02.2010, p. 129-134.

Research output: Contribution to journalArticle

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