Clonal diversity of methicillin-resistant staphylococcus aureus in UKM medical centre: Characterisation by multilocus sequence typing of different SCCmec type representatives

Muttaqillah Najihan Abdul Samat, Salasawati Hussin, Hui Min Neoh, Ainihayati Noordin, Chuan Hun Ding, Asrul Abdul Wahab, Md. Mostafizur Rahman

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Abstract

Multilocus sequence typing (MLST) has been used to characterise methicillin-resistant Staphylococcus aureus (MRSA) isolates into sequence types (STs) and together with SCCmec typing, form the clonal nomenclature for MRSA. MLST was conducted as per the standard protocol on ten out of 236 isolates collected previously from January to December 2009 representing four different SCCmec types. Relationship analysis was performed with eBURST via the MLST website. Four unlinked 'singleton' STs were detected: ST30, ST239, ST772 and ST1178. Together with SCCmec typing, five MRSA clones were identified: ST30-IV, ST239-II, ST239-III, ST772-V and ST1178-IV. Clones ST239-III and ST30-IV are already established in Malaysian hospitals and in the local community, respectively. ST772-V is an emerging clone reported previously to have a propensity to displace pre-existing predominant clones. A clone involving the predominant ST in Malaysia (ST239) with SCCmec type II is the first of its kind to be identified. MRSA clones in our centre are very diverse and clone surveillance with large sample sizes should be undertaken as collaborative efforts between local institutions to maximise detection coverage.

Original languageEnglish
Pages (from-to)1315-1323
Number of pages9
JournalSains Malaysiana
Volume44
Issue number9
Publication statusPublished - 1 Sep 2015

Fingerprint

Multilocus Sequence Typing
Methicillin-Resistant Staphylococcus aureus
Clone Cells
Malaysia
Terminology
Sample Size

Keywords

  • Methicillin-resistant stafilokokal aureus (MRSA)
  • Molecular characterisation
  • Multilocus sequence typing (MLST)
  • Staphylococcus cassette chromosome mec (SCCmec)

ASJC Scopus subject areas

  • General

Cite this

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title = "Clonal diversity of methicillin-resistant staphylococcus aureus in UKM medical centre: Characterisation by multilocus sequence typing of different SCCmec type representatives",
abstract = "Multilocus sequence typing (MLST) has been used to characterise methicillin-resistant Staphylococcus aureus (MRSA) isolates into sequence types (STs) and together with SCCmec typing, form the clonal nomenclature for MRSA. MLST was conducted as per the standard protocol on ten out of 236 isolates collected previously from January to December 2009 representing four different SCCmec types. Relationship analysis was performed with eBURST via the MLST website. Four unlinked 'singleton' STs were detected: ST30, ST239, ST772 and ST1178. Together with SCCmec typing, five MRSA clones were identified: ST30-IV, ST239-II, ST239-III, ST772-V and ST1178-IV. Clones ST239-III and ST30-IV are already established in Malaysian hospitals and in the local community, respectively. ST772-V is an emerging clone reported previously to have a propensity to displace pre-existing predominant clones. A clone involving the predominant ST in Malaysia (ST239) with SCCmec type II is the first of its kind to be identified. MRSA clones in our centre are very diverse and clone surveillance with large sample sizes should be undertaken as collaborative efforts between local institutions to maximise detection coverage.",
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AU - Abdul Samat, Muttaqillah Najihan

AU - Hussin, Salasawati

AU - Neoh, Hui Min

AU - Noordin, Ainihayati

AU - Ding, Chuan Hun

AU - Abdul Wahab, Asrul

AU - Rahman, Md. Mostafizur

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