Chondrogenesis of adipose-derived stem cells with chondrocytes in low serum towards clinical application

Adila A. Hamid, Satish Vaarman Jeyabalan, Aleza Omar, Nik Zattil Hanan Mohd Yasin, Wong Tzeng Lin, Liau Ling Ling, Nur Azurah Abdul Ghani, Aneeza Khairiyah Wan Hamizan, Chua Kien Hui

Research output: Contribution to journalArticle

Abstract

Currently, fetal bovine serum (FBS) have been widely use in culture media to promote human cell proliferation. However, the usage of FBS for cell therapy in clinical application was associated with the risk of viral and prion transmission as well as immune rejection. To provide an option for this risk, this study was conducted to determine the effect of adipose derived stem cells (ADSCs) co-culture with chondrocyte in promoting cell proliferation and chondrogenesis toward FBS free condition. ADSCs co-cultured with chondrocyte at the ratio of 1:1, 2:1 and 1:2 were tested. Cell morphology changes, cell proliferation and gene expression level of stemness (Oct4, FGF-4, Nanog) and chondrogenic (Collagen Type II, ACP) were assessed. The results showed ADSCs in all co-culture groups changed morphology from fibroblastic spindle to polygonal shape which resembled chondrocytes. The morphological changes were accompanied with increased expression of chondrogenic genes; denoted chondrogenesis process. While maintaining expression of stemness genes indicated continuation of cell proliferation. From the three co-culture groups tested; ADSCs and chondrocytes (1:1 ratio) have been shown to exert better effects in promoting cell proliferation and chondrogenesis. In conclusion, ADSCs could replace FBS to grow sufficient number of chondrogenic cells to repair cartilage injury in the near future. Further in vivo study should be performed to test the effectiveness of this co-culture technique in cartilage injury repair.

Original languageEnglish
Pages (from-to)2369-2379
Number of pages11
JournalSains Malaysiana
Volume47
Issue number10
DOIs
Publication statusPublished - 1 Oct 2018

Fingerprint

Chondrogenesis
Chondrocytes
Coculture Techniques
Stem Cells
Cell Proliferation
Serum
Gene Expression
Cartilage
Culture Techniques
Collagen Type II
Prions
Wounds and Injuries
Cell- and Tissue-Based Therapy
Culture Media
Cell Culture Techniques
Cell Count

Keywords

  • Cartilage
  • Chondrocyte
  • Clinical application
  • Serum
  • Stem cell

ASJC Scopus subject areas

  • General

Cite this

Chondrogenesis of adipose-derived stem cells with chondrocytes in low serum towards clinical application. / A. Hamid, Adila; Jeyabalan, Satish Vaarman; Omar, Aleza; Yasin, Nik Zattil Hanan Mohd; Lin, Wong Tzeng; Ling, Liau Ling; Abdul Ghani, Nur Azurah; Wan Hamizan, Aneeza Khairiyah; Kien Hui, Chua.

In: Sains Malaysiana, Vol. 47, No. 10, 01.10.2018, p. 2369-2379.

Research output: Contribution to journalArticle

A. Hamid, Adila ; Jeyabalan, Satish Vaarman ; Omar, Aleza ; Yasin, Nik Zattil Hanan Mohd ; Lin, Wong Tzeng ; Ling, Liau Ling ; Abdul Ghani, Nur Azurah ; Wan Hamizan, Aneeza Khairiyah ; Kien Hui, Chua. / Chondrogenesis of adipose-derived stem cells with chondrocytes in low serum towards clinical application. In: Sains Malaysiana. 2018 ; Vol. 47, No. 10. pp. 2369-2379.
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