Choice of PCR microtube can impact on the success of long-range PCRs

Eng Wee Chua, Allison L. Miller, Martin A. Kennedy

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We compared four brands of microtubes with respect to their suitability for long-range polymerase chain reactions (PCRs). One of the four brands was found to have an inhibitory effect, decreasing PCR yields. The effect was universal across different PCR or enzyme systems. Increased ultraviolet absorbance suggests leaching of unknown chemical species into PCR mixtures. However, this could not be confirmed by high-performance liquid chromatography-mass spectrometry analysis. Nevertheless, our article demonstrates a clear impact of the choice of microtubes on long-range PCR success. Due consideration should be given to the PCR microtubes when determining optimal reaction conditions for long-range PCR.

Original languageEnglish
Pages (from-to)115-117
Number of pages3
JournalAnalytical Biochemistry
Volume477
DOIs
Publication statusPublished - 15 May 2015

Fingerprint

Polymerase chain reaction
Polymerase Chain Reaction
High performance liquid chromatography
Leaching
Mass spectrometry
Mass Spectrometry
High Pressure Liquid Chromatography
Enzymes

Keywords

  • Chemical leachate
  • Contaminants
  • CYP2D6
  • Long-range PCR
  • PCR inhibition
  • PCR microtubes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Cell Biology

Cite this

Choice of PCR microtube can impact on the success of long-range PCRs. / Chua, Eng Wee; Miller, Allison L.; Kennedy, Martin A.

In: Analytical Biochemistry, Vol. 477, 15.05.2015, p. 115-117.

Research output: Contribution to journalArticle

Chua, Eng Wee ; Miller, Allison L. ; Kennedy, Martin A. / Choice of PCR microtube can impact on the success of long-range PCRs. In: Analytical Biochemistry. 2015 ; Vol. 477. pp. 115-117.
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