Chlorella vulgaris triggers apoptosis in hepatocarcinogenesis-induced rats

Emey Suhana Mohd Azamai, Suhaniza Sulaiman, Shafina Hanim Mohd Habib, Mee Lee Looi, Srijit Das, Nor Aini Abdul Hamid, Wan Zurinah Wan Ngah, Yasmin Anum Mohd Yusof

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Chlorella vulgaris (CV) has been reported to have antioxidant and anticancer properties. We evaluated the effect of CV on apoptotic regulator protein expression in liver cancer-induced rats. Male Wistar rats (200∼250 g) were divided into eight groups: control group (normal diet), CDE group (choline deficient diet supplemented with ethionine in drinking water to induce hepatocarcinogenesis), CV groups with three different doses of CV (50, 150, and 300 mg/kg body weight), and CDE groups treated with different doses of CV (50, 150, and 300 mg/ kg body weight). Rats were sacrificed at various weeks and liver tissues were embedded in paraffin blocks for immunohistochemistry studies. CV, at increasing doses, decreased the expression of anti-apoptotic protein, Bcl-2, but increased the expression of pro-apoptotic protein, caspase 8, in CDE rats, which was correlated with decreased hepatoctyes proliferation and increased apoptosis as determined by bromodeoxy-uridine (BrdU) labeling and terminal deoxynucleotidyl transferase mediated dUTP nick-end labeling (TUNEL) assay, respectively. Our study shows that CV has definite chemopreventive effect by inducing apoptosis via decreasing the expression of Bcl-2 and increasing the expression of caspase 8 in hepatocarcinogenesis-induced rats.

Original languageEnglish
Pages (from-to)14-21
Number of pages8
JournalJournal of Zhejiang University: Science B
Volume10
Issue number1
DOIs
Publication statusPublished - 2009

Fingerprint

Chlorella vulgaris
Rats
apoptosis
Apoptosis
rats
Apoptosis Regulatory Proteins
Caspase 8
Nutrition
Liver
caspase-8
Labeling
Ethionine
DNA Nucleotidylexotransferase
Uridine
dosage
Body Weight
ethionine
Choline
Diet
Drinking Water

Keywords

  • Apoptosis
  • Bcl-2
  • Caspase8
  • Chlorella vulgaris (CV)
  • Livercancer

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • veterinary(all)

Cite this

Mohd Azamai, E. S., Sulaiman, S., Mohd Habib, S. H., Looi, M. L., Das, S., Abdul Hamid, N. A., ... Mohd Yusof, Y. A. (2009). Chlorella vulgaris triggers apoptosis in hepatocarcinogenesis-induced rats. Journal of Zhejiang University: Science B, 10(1), 14-21. https://doi.org/10.1631/jzus.B0820168

Chlorella vulgaris triggers apoptosis in hepatocarcinogenesis-induced rats. / Mohd Azamai, Emey Suhana; Sulaiman, Suhaniza; Mohd Habib, Shafina Hanim; Looi, Mee Lee; Das, Srijit; Abdul Hamid, Nor Aini; Wan Ngah, Wan Zurinah; Mohd Yusof, Yasmin Anum.

In: Journal of Zhejiang University: Science B, Vol. 10, No. 1, 2009, p. 14-21.

Research output: Contribution to journalArticle

Mohd Azamai, ES, Sulaiman, S, Mohd Habib, SH, Looi, ML, Das, S, Abdul Hamid, NA, Wan Ngah, WZ & Mohd Yusof, YA 2009, 'Chlorella vulgaris triggers apoptosis in hepatocarcinogenesis-induced rats', Journal of Zhejiang University: Science B, vol. 10, no. 1, pp. 14-21. https://doi.org/10.1631/jzus.B0820168
Mohd Azamai, Emey Suhana ; Sulaiman, Suhaniza ; Mohd Habib, Shafina Hanim ; Looi, Mee Lee ; Das, Srijit ; Abdul Hamid, Nor Aini ; Wan Ngah, Wan Zurinah ; Mohd Yusof, Yasmin Anum. / Chlorella vulgaris triggers apoptosis in hepatocarcinogenesis-induced rats. In: Journal of Zhejiang University: Science B. 2009 ; Vol. 10, No. 1. pp. 14-21.
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