Chitosan-based tyrosinase optical phenol biosensor employing hybrid nafion/sol-gel silicate for MBTH immobilization

Jaafar Abdullah, Musa Ahmad, Yook Heng Lee, Nadarajah Karuppiah, Hamidah Sidek

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

The development of an optical biosensor based on immobilization of 3-methyl-2-benzothiazolinone hydrazone (MBTH) in hybrid nafion/sol-gel silicate film and tyrosinase in chitosan film for the detection of phenolic compounds has been described. Tyrosinase was immobilized in chitosan film deposited on the hybrid nafion/sol-gel silicate film containing MBTH. The enzymatic oxidation product of phenolic compounds were stabilized through formation of adduct with MBTH to produce a maroon color adduct. The color intensity of adduct was found to increase proportionally with the increase of the substrate concentrations after 5 min exposure. The linearity of the biosensor towards phenol, catechol and m-cresol were in the respective concentration range of 0.5-7.0, 0.5-10.0 and 1.0-13.0 mg/L with detection limit of 0.18, 0.23 and 0.43 mg/L, respectively. The biosensor shows a good stability for at least 3 months.

Original languageEnglish
Pages (from-to)527-532
Number of pages6
JournalTalanta
Volume70
Issue number3
DOIs
Publication statusPublished - 15 Oct 2006

Fingerprint

Hydrazones
Silicates
Monophenol Monooxygenase
Chitosan
Biosensing Techniques
Polymethyl Methacrylate
Phenol
Biosensors
Immobilization
Sol-gels
Gels
Color
Limit of Detection
Oxidation
perfluorosulfonic acid
Substrates

Keywords

  • Chitosan
  • Hybrid nafion/sol-gel silicate
  • Optical biosensor
  • Phenols
  • Tyrosinase

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

Cite this

Chitosan-based tyrosinase optical phenol biosensor employing hybrid nafion/sol-gel silicate for MBTH immobilization. / Abdullah, Jaafar; Ahmad, Musa; Lee, Yook Heng; Karuppiah, Nadarajah; Sidek, Hamidah.

In: Talanta, Vol. 70, No. 3, 15.10.2006, p. 527-532.

Research output: Contribution to journalArticle

Abdullah, Jaafar ; Ahmad, Musa ; Lee, Yook Heng ; Karuppiah, Nadarajah ; Sidek, Hamidah. / Chitosan-based tyrosinase optical phenol biosensor employing hybrid nafion/sol-gel silicate for MBTH immobilization. In: Talanta. 2006 ; Vol. 70, No. 3. pp. 527-532.
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