Chironomus group classification according to the mapping of polytene chromosomes

Syafinaz Salleh, Ahmad Abas Kutty

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Chironomus is one of the important genera in Chironomidae family since they are widely diverse and abundance in aquatic ecosystem. Since Chironomus is very diverse, taxonomic work on this genus is very difficult and incomplete. Objective of this study is to form group classification of Chironomus according to the polytene chromosome mapping. The specific characteristics of polytene chromosomes in the salivary gland appeared to be particularly promising for taxonomic diagnosis of chironomid species. Chironomid larvae were collected from pristine sites at Sg. Langat and cultured in laboratory to reach fourth instar stage. The salivary glands were removed from larvae and chromosomes were stained with aceto orcein. Results showed that polytene chromosomes of Chironomus comprise of three long metacentric or submetacentric arms (BF, CD and AE arms) and one short acrocentric (G arm). In regards to nucleolar organizing region (NOR), Balbiani ring (BR), puffings and chromosome rearrangement, a number of four groups of different banding patterns were found. Two groups called as G group A and B have common NOR on arm BF and BR on arm G. However, group A has rearrangement pattern on arm CD and not in group B. This makes group B separated from group A. Another two groups called as groups C and D do not have common NOR on arm BF and also BR on arm G. Groups C and D were separated using arms G and arm AE. At arm G, only group C rearrangement pattern at unit 23c whereas group D was found to have large NOR at arm G and as well as arm AE, only group D has rearrangement pattern at unit 12c. This study indicates that chromosome arrangement could aid in revealing Chironomus diversity.

Original languageEnglish
Title of host publicationAIP Conference Proceedings
Pages614-619
Number of pages6
Volume1571
DOIs
Publication statusPublished - 2013
Event2013 UKM Faculty of Science and Technology Post-Graduate Colloquium - Selangor
Duration: 3 Jul 20134 Jul 2013

Other

Other2013 UKM Faculty of Science and Technology Post-Graduate Colloquium
CitySelangor
Period3/7/134/7/13

Fingerprint

chromosomes
organizing
larvae
salivary glands
rings
ecosystems

Keywords

  • Acrocentric
  • Balbiani ring
  • Chironomus
  • Metacentric
  • Submetacentric

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Chironomus group classification according to the mapping of polytene chromosomes. / Salleh, Syafinaz; Abas Kutty, Ahmad.

AIP Conference Proceedings. Vol. 1571 2013. p. 614-619.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Salleh, S & Abas Kutty, A 2013, Chironomus group classification according to the mapping of polytene chromosomes. in AIP Conference Proceedings. vol. 1571, pp. 614-619, 2013 UKM Faculty of Science and Technology Post-Graduate Colloquium, Selangor, 3/7/13. https://doi.org/10.1063/1.4858722
Salleh, Syafinaz ; Abas Kutty, Ahmad. / Chironomus group classification according to the mapping of polytene chromosomes. AIP Conference Proceedings. Vol. 1571 2013. pp. 614-619
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