Chimeric Newcastle disease virus nucleocapsid with parts of viral hemagglutinin-neuraminidase and fusion proteins

A. Rabu, W. S. Tan, C. L. Kho, A. R. Omar, K. Yusoff

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The nucleocapsid (NP) protein of Newcastle disease virus (NDV) self-assembled in Escherichia coli as ring-like and herringbone-like particles. Several chimeric NP proteins were constructed in which the antigenic regions of the hemagglutinin-neuraminidase (HN) and fusion (F) proteins of NDV, myc epitope, and six histidines (a hexa-His tag) were linked to the C-terminus of the NP monomer. These chimeric proteins were expressed efficiently in soluble form in E. coli as detected by Western blot analysis. Electron microscopy of the purified products revealed that they self-assembled into ring-like particles. These chimeric particles exhibited antigenicity of the myc epitope, suggesting that the foreign sequences were exposed on the surface of the particles. Chickens inoculated with the chimeric particles mounted an immune response against NDV, suggesting the possibility of use of the ring-like particle as a carrier of immunogens in subunit vaccines and immunological reagents.

Original languageEnglish
Pages (from-to)211-217
Number of pages7
JournalActa Virologica
Volume46
Issue number4
Publication statusPublished - 2002
Externally publishedYes

Fingerprint

Viral Hemagglutinins
Nucleocapsid
Newcastle disease virus
Neuraminidase
Epitopes
Viral Fusion Proteins
Escherichia coli
Nucleocapsid Proteins
Subunit Vaccines
Hemagglutinins
Histidine
Chickens
Electron Microscopy
Proteins
Western Blotting

Keywords

  • Chimeric NP protein
  • Molecular carrier
  • NDV
  • Nucleocapsid
  • Protein assembly

ASJC Scopus subject areas

  • Virology
  • Immunology

Cite this

Rabu, A., Tan, W. S., Kho, C. L., Omar, A. R., & Yusoff, K. (2002). Chimeric Newcastle disease virus nucleocapsid with parts of viral hemagglutinin-neuraminidase and fusion proteins. Acta Virologica, 46(4), 211-217.

Chimeric Newcastle disease virus nucleocapsid with parts of viral hemagglutinin-neuraminidase and fusion proteins. / Rabu, A.; Tan, W. S.; Kho, C. L.; Omar, A. R.; Yusoff, K.

In: Acta Virologica, Vol. 46, No. 4, 2002, p. 211-217.

Research output: Contribution to journalArticle

Rabu, A, Tan, WS, Kho, CL, Omar, AR & Yusoff, K 2002, 'Chimeric Newcastle disease virus nucleocapsid with parts of viral hemagglutinin-neuraminidase and fusion proteins', Acta Virologica, vol. 46, no. 4, pp. 211-217.
Rabu, A. ; Tan, W. S. ; Kho, C. L. ; Omar, A. R. ; Yusoff, K. / Chimeric Newcastle disease virus nucleocapsid with parts of viral hemagglutinin-neuraminidase and fusion proteins. In: Acta Virologica. 2002 ; Vol. 46, No. 4. pp. 211-217.
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