Chemopreventive and immunomodulatory effects of Murraya koenigii aqueous extract on 4T1 breast cancer cell-challenged mice

Swee Keong Yeap, Nadiah Abu, Nurul Elyani Mohamad, Boon Kee Beh, Wan Yong Ho, Siamak Ebrahimi, Hamidah Mohd Yusof, Huynh Ky, Sheau Wei Tan, Noorjahan Banu Alitheen

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Background: The progression of breast cancer is increasing at an alarming rate, particularly in western countries. Meanwhile, the lower incidence in Asian countries could be attributed to the heavy incorporation of green leaves vegetables or spices in their diets. Murraya koenigii (MK) or often times known as curry leaves are common spice used mostly in tropical countries. Anti-inflammatory and chemopreventive effects of MK aqueous extract on 4T1 breast cancer cell-challenged mice were evaluated. Methods: Herein, cytotoxic activity of MK was first tested on 4T1 cells in vitroby MTT assay. Then, in vivo chemopreventive study was conducted where mice were fed with extracts prior to and after inducing the tumor (inoculation). Tumor size was monitored post-4T1 inoculation. At the end of experiment, histopathology of tumor sections, T cell immunophenotyping, tumor nitric oxide level, serum cytokine level and qPCR analysis on expression of iNOS, iCAM, NF-kB and c-MYC were performed. Results: MK reduced the tumors' size and lung metastasis aside from inhibited the viability of 4T1 cells in vitro. Furthermore, it decreased the level of nitric oxide and inflammation-related cytokines and genes, including iNOS, iCAM, NF-kB and c-MYC. Conclusion: The results propose that, MK managed to inhibit the progression of tumor via immunostimulatory effect and inflammatory reaction within the tumor samples. This suggests that MKconsumption could be a savior in the search of new chemopreventive agents.

Original languageEnglish
Article number306
JournalBMC Complementary and Alternative Medicine
Volume15
Issue number1
DOIs
Publication statusPublished - 4 Sep 2015
Externally publishedYes

Fingerprint

Murraya
Breast Neoplasms
Neoplasms
Spices
NF-kappa B
Nitric Oxide
Cytokines
Immunophenotyping
Vegetables
Cell Survival
Anti-Inflammatory Agents
Neoplasm Metastasis
Diet
Inflammation
T-Lymphocytes
Lung
Incidence

Keywords

  • 4T1 cells
  • Anti-inflammation
  • Chemopreventive
  • Curry leaves
  • Murraya koenigii

ASJC Scopus subject areas

  • Complementary and alternative medicine

Cite this

Chemopreventive and immunomodulatory effects of Murraya koenigii aqueous extract on 4T1 breast cancer cell-challenged mice. / Yeap, Swee Keong; Abu, Nadiah; Mohamad, Nurul Elyani; Beh, Boon Kee; Ho, Wan Yong; Ebrahimi, Siamak; Yusof, Hamidah Mohd; Ky, Huynh; Tan, Sheau Wei; Alitheen, Noorjahan Banu.

In: BMC Complementary and Alternative Medicine, Vol. 15, No. 1, 306, 04.09.2015.

Research output: Contribution to journalArticle

Yeap, Swee Keong ; Abu, Nadiah ; Mohamad, Nurul Elyani ; Beh, Boon Kee ; Ho, Wan Yong ; Ebrahimi, Siamak ; Yusof, Hamidah Mohd ; Ky, Huynh ; Tan, Sheau Wei ; Alitheen, Noorjahan Banu. / Chemopreventive and immunomodulatory effects of Murraya koenigii aqueous extract on 4T1 breast cancer cell-challenged mice. In: BMC Complementary and Alternative Medicine. 2015 ; Vol. 15, No. 1.
@article{f8304799bf714085943bd829dfee4d34,
title = "Chemopreventive and immunomodulatory effects of Murraya koenigii aqueous extract on 4T1 breast cancer cell-challenged mice",
abstract = "Background: The progression of breast cancer is increasing at an alarming rate, particularly in western countries. Meanwhile, the lower incidence in Asian countries could be attributed to the heavy incorporation of green leaves vegetables or spices in their diets. Murraya koenigii (MK) or often times known as curry leaves are common spice used mostly in tropical countries. Anti-inflammatory and chemopreventive effects of MK aqueous extract on 4T1 breast cancer cell-challenged mice were evaluated. Methods: Herein, cytotoxic activity of MK was first tested on 4T1 cells in vitroby MTT assay. Then, in vivo chemopreventive study was conducted where mice were fed with extracts prior to and after inducing the tumor (inoculation). Tumor size was monitored post-4T1 inoculation. At the end of experiment, histopathology of tumor sections, T cell immunophenotyping, tumor nitric oxide level, serum cytokine level and qPCR analysis on expression of iNOS, iCAM, NF-kB and c-MYC were performed. Results: MK reduced the tumors' size and lung metastasis aside from inhibited the viability of 4T1 cells in vitro. Furthermore, it decreased the level of nitric oxide and inflammation-related cytokines and genes, including iNOS, iCAM, NF-kB and c-MYC. Conclusion: The results propose that, MK managed to inhibit the progression of tumor via immunostimulatory effect and inflammatory reaction within the tumor samples. This suggests that MKconsumption could be a savior in the search of new chemopreventive agents.",
keywords = "4T1 cells, Anti-inflammation, Chemopreventive, Curry leaves, Murraya koenigii",
author = "Yeap, {Swee Keong} and Nadiah Abu and Mohamad, {Nurul Elyani} and Beh, {Boon Kee} and Ho, {Wan Yong} and Siamak Ebrahimi and Yusof, {Hamidah Mohd} and Huynh Ky and Tan, {Sheau Wei} and Alitheen, {Noorjahan Banu}",
year = "2015",
month = "9",
day = "4",
doi = "10.1186/s12906-015-0832-z",
language = "English",
volume = "15",
journal = "BMC Complementary and Alternative Medicine",
issn = "1472-6882",
publisher = "BioMed Central",
number = "1",

}

TY - JOUR

T1 - Chemopreventive and immunomodulatory effects of Murraya koenigii aqueous extract on 4T1 breast cancer cell-challenged mice

AU - Yeap, Swee Keong

AU - Abu, Nadiah

AU - Mohamad, Nurul Elyani

AU - Beh, Boon Kee

AU - Ho, Wan Yong

AU - Ebrahimi, Siamak

AU - Yusof, Hamidah Mohd

AU - Ky, Huynh

AU - Tan, Sheau Wei

AU - Alitheen, Noorjahan Banu

PY - 2015/9/4

Y1 - 2015/9/4

N2 - Background: The progression of breast cancer is increasing at an alarming rate, particularly in western countries. Meanwhile, the lower incidence in Asian countries could be attributed to the heavy incorporation of green leaves vegetables or spices in their diets. Murraya koenigii (MK) or often times known as curry leaves are common spice used mostly in tropical countries. Anti-inflammatory and chemopreventive effects of MK aqueous extract on 4T1 breast cancer cell-challenged mice were evaluated. Methods: Herein, cytotoxic activity of MK was first tested on 4T1 cells in vitroby MTT assay. Then, in vivo chemopreventive study was conducted where mice were fed with extracts prior to and after inducing the tumor (inoculation). Tumor size was monitored post-4T1 inoculation. At the end of experiment, histopathology of tumor sections, T cell immunophenotyping, tumor nitric oxide level, serum cytokine level and qPCR analysis on expression of iNOS, iCAM, NF-kB and c-MYC were performed. Results: MK reduced the tumors' size and lung metastasis aside from inhibited the viability of 4T1 cells in vitro. Furthermore, it decreased the level of nitric oxide and inflammation-related cytokines and genes, including iNOS, iCAM, NF-kB and c-MYC. Conclusion: The results propose that, MK managed to inhibit the progression of tumor via immunostimulatory effect and inflammatory reaction within the tumor samples. This suggests that MKconsumption could be a savior in the search of new chemopreventive agents.

AB - Background: The progression of breast cancer is increasing at an alarming rate, particularly in western countries. Meanwhile, the lower incidence in Asian countries could be attributed to the heavy incorporation of green leaves vegetables or spices in their diets. Murraya koenigii (MK) or often times known as curry leaves are common spice used mostly in tropical countries. Anti-inflammatory and chemopreventive effects of MK aqueous extract on 4T1 breast cancer cell-challenged mice were evaluated. Methods: Herein, cytotoxic activity of MK was first tested on 4T1 cells in vitroby MTT assay. Then, in vivo chemopreventive study was conducted where mice were fed with extracts prior to and after inducing the tumor (inoculation). Tumor size was monitored post-4T1 inoculation. At the end of experiment, histopathology of tumor sections, T cell immunophenotyping, tumor nitric oxide level, serum cytokine level and qPCR analysis on expression of iNOS, iCAM, NF-kB and c-MYC were performed. Results: MK reduced the tumors' size and lung metastasis aside from inhibited the viability of 4T1 cells in vitro. Furthermore, it decreased the level of nitric oxide and inflammation-related cytokines and genes, including iNOS, iCAM, NF-kB and c-MYC. Conclusion: The results propose that, MK managed to inhibit the progression of tumor via immunostimulatory effect and inflammatory reaction within the tumor samples. This suggests that MKconsumption could be a savior in the search of new chemopreventive agents.

KW - 4T1 cells

KW - Anti-inflammation

KW - Chemopreventive

KW - Curry leaves

KW - Murraya koenigii

UR - http://www.scopus.com/inward/record.url?scp=84941315899&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84941315899&partnerID=8YFLogxK

U2 - 10.1186/s12906-015-0832-z

DO - 10.1186/s12906-015-0832-z

M3 - Article

VL - 15

JO - BMC Complementary and Alternative Medicine

JF - BMC Complementary and Alternative Medicine

SN - 1472-6882

IS - 1

M1 - 306

ER -