Chemometrics-Assisted Shotgun Proteomics for Establishment of Potential Peptide Markers of Non-Halal Pork (Sus scrofa) among Halal Beef and Chicken

Mohd Hafis Yuswan, Wan Mohd Aizat Wan Kamaruddin, Ahmad Afifullah Lokman, Mohd Nasir Mohd Desa, Shuhaimi Mustafa, Nadiah Mat Junoh, Zetty Norhana Balia Yusof, Rozi Mohamed, Zulfawwaz Mohmad, Dhilia Udie Lamasudin

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Authentication of meat is a global concern owing to meat adulteration with pork; however, no study, to date, has established a method to identify species-specific peptide markers for ascertaining adulterated meat. The present study aimed to describe chemometrics-assisted shotgun proteomics, which has recently been reported to establish potential peptide markers for non-Halal pork among Halal beef and chicken meat. To identify the potential peptide markers through peptide mass fingerprinting (PMF) analysis, chemometric analysis comprised principal component analysis and orthogonal partial least square-discriminant analysis, both of which proved statistically useful. Subsequently, through targeted tandem liquid chromatography-mass spectrometry (LC-MS) analysis, the primary structure of the identified peptide markers was revealed using the de novo identification approach. A decoy, randomised and concatenated database search program comprising MS-Fit and MS-Tag was used for PMF and targeted tandem LC-MS analysis, respectively. Results obtained using both PMF and targeted tandem LC-MS analysis complemented one another, indicating that these methods can yield consistent results to identify peptide markers together with chemometric analysis.

Original languageEnglish
JournalFood Analytical Methods
DOIs
Publication statusAccepted/In press - 1 Jan 2018

Fingerprint

Beef
Sus scrofa
chemometrics
Firearms
Proteomics
proteomics
Peptides
pork
Chickens
beef
Meat
Peptide Mapping
peptides
chickens
Meats
Liquid Chromatography
Mass Spectrometry
Liquid chromatography
liquid chromatography
Mass spectrometry

Keywords

  • Chemometrics
  • Non-Halal pork
  • Peptide markers
  • Peptide mass fingerprinting
  • Shotgun proteomics
  • Targeted tandem LC-MS

ASJC Scopus subject areas

  • Analytical Chemistry
  • Food Science
  • Applied Microbiology and Biotechnology
  • Safety, Risk, Reliability and Quality
  • Safety Research

Cite this

Chemometrics-Assisted Shotgun Proteomics for Establishment of Potential Peptide Markers of Non-Halal Pork (Sus scrofa) among Halal Beef and Chicken. / Yuswan, Mohd Hafis; Wan Kamaruddin, Wan Mohd Aizat; Lokman, Ahmad Afifullah; Desa, Mohd Nasir Mohd; Mustafa, Shuhaimi; Junoh, Nadiah Mat; Yusof, Zetty Norhana Balia; Mohamed, Rozi; Mohmad, Zulfawwaz; Lamasudin, Dhilia Udie.

In: Food Analytical Methods, 01.01.2018.

Research output: Contribution to journalArticle

Yuswan, Mohd Hafis ; Wan Kamaruddin, Wan Mohd Aizat ; Lokman, Ahmad Afifullah ; Desa, Mohd Nasir Mohd ; Mustafa, Shuhaimi ; Junoh, Nadiah Mat ; Yusof, Zetty Norhana Balia ; Mohamed, Rozi ; Mohmad, Zulfawwaz ; Lamasudin, Dhilia Udie. / Chemometrics-Assisted Shotgun Proteomics for Establishment of Potential Peptide Markers of Non-Halal Pork (Sus scrofa) among Halal Beef and Chicken. In: Food Analytical Methods. 2018.
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