Chemical profiling and quantification of tannins in Phyllanthus niruri Linn. fractionated by SFE method

Masturah Markom, Masitah Hasan, Wan Ramli Wan Daud, Nurina Anuar, Osman Hassan, Harcharan Singh

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Chemical profiles or fingerprints of polyphenolic compounds (condensed and hydrolyzable tannins) in various fractions of Phyllanthus niruri Linn extracted using supercritical carbon dioxide and various polar cosolvents, namely water, methanol, and ethanol are presented. Chemical analysis of the extracted fractions was undertaken using High Performance Liquid Chromatography (HPLC) with the in-house method. Good peak reproducibility of intra-day (R.S.D range 0.01-0.21 min) and inter-day (R.S.D range 0.5-0.8 min) was obtained for the detection of ellagitannins (hydrolyzable tannins) and flavonoids (condensed tannins). Fractions extracted using ethanol-water mixtures as cosolvent at 200 bar and 60°C exhibited an appealing behavior whereby non-polar compounds and flavonoids were able to be fractionated before the extraction of ellagitannins. Contents of three major ellagitannins, namely gallic acid (0.39-0.48% g/g), corilagin (2.42-3.00% g/g), and ellagic acid (5.94-6.48% g/g), were relatively higher compared to the commercial HEPAR-P™ standardized extract (0.21, 2.64, and 4.17% g/g, respectively). The study shows that the supercritical fluid extraction (SFE) method with the use of appropriate cosolvents is able to produce P. niruri fractions with improved yields and different chemical characteristic, which thus can be used as a rapid preparative tool for further downstream processing of plant samples.

Original languageEnglish
Pages (from-to)71-78
Number of pages8
JournalSeparation Science and Technology
Volume46
Issue number1
DOIs
Publication statusPublished - Jan 2011

Fingerprint

Hydrolyzable Tannins
Tannins
Flavonoids
Supercritical fluids
Ellagic acid
Polyphenolic compounds
Ethanol
High performance liquid chromatography
Proanthocyanidins
Water
Carbon dioxide
Methanol
Acids
Ellagic Acid
Processing
Chemical analysis
Gallic Acid
Carbon Dioxide

Keywords

  • Chemical analysis
  • Fractionation
  • Phyllanthus niruri
  • Polar cosolvents
  • Supercritical carbon dioxide

ASJC Scopus subject areas

  • Chemistry(all)
  • Process Chemistry and Technology
  • Chemical Engineering(all)
  • Filtration and Separation

Cite this

Chemical profiling and quantification of tannins in Phyllanthus niruri Linn. fractionated by SFE method. / Markom, Masturah; Hasan, Masitah; Wan Daud, Wan Ramli; Anuar, Nurina; Hassan, Osman; Singh, Harcharan.

In: Separation Science and Technology, Vol. 46, No. 1, 01.2011, p. 71-78.

Research output: Contribution to journalArticle

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