Characterization study of a highly specific affinity membrane for trypsin purification

Sofiah Hamzah, Nora’Aini Ali, Marinah Mohd Ariffin, Abdul Wahab Mohammad

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This study aimed to characterize a highly specific affinity membrane for trypsin separation. The basic membrane was fabricated using 15% polysulfone via phase inversion technique. Membrane surface modification was employed by immersing the native membrane into the chitosan solution for 60 minutes dip times. Further modification was performed to activate the PSf/chitosan hybrid membrane using glutaraldehyde before ligand immobilization. The prepared affinity membrane was characterized in term of morphology using scanning electron microscope and the presence of aldehyde group and ovomucoid were confirmed by ATR-FTIR. Performance of affinity membrane was valuted by adsorption study of trypsin enzyme. The observed results show that the developed affinity membrane, with 0.7 mg/ml ligands was able to adsorb trypsin for about 0.219 mg/cm2 membrane

Original languageEnglish
Title of host publicationApplied Mechanics and Materials
PublisherTrans Tech Publications Ltd
Pages592-595
Number of pages4
Volume625
ISBN (Print)9783038351818
DOIs
Publication statusPublished - 2014
Event3rd International Conference on Process Engineering and Advanced Materials, ICPEAM 2014 - Kuala Lumpur
Duration: 3 Jun 20145 Jun 2014

Publication series

NameApplied Mechanics and Materials
Volume625
ISSN (Print)16609336
ISSN (Electronic)16627482

Other

Other3rd International Conference on Process Engineering and Advanced Materials, ICPEAM 2014
CityKuala Lumpur
Period3/6/145/6/14

Fingerprint

Purification
Membranes
Chitosan
Ligands
Polysulfones
Aldehydes
Surface treatment
Electron microscopes
Enzymes
Scanning
Adsorption

Keywords

  • Affinity
  • Membrane
  • Trypsin
  • Trypsin purification
  • Ultrafiltration

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Hamzah, S., Ali, NA., Ariffin, M. M., & Mohammad, A. W. (2014). Characterization study of a highly specific affinity membrane for trypsin purification. In Applied Mechanics and Materials (Vol. 625, pp. 592-595). (Applied Mechanics and Materials; Vol. 625). Trans Tech Publications Ltd. https://doi.org/10.4028/www.scientific.net/AMM.625.592

Characterization study of a highly specific affinity membrane for trypsin purification. / Hamzah, Sofiah; Ali, Nora’Aini; Ariffin, Marinah Mohd; Mohammad, Abdul Wahab.

Applied Mechanics and Materials. Vol. 625 Trans Tech Publications Ltd, 2014. p. 592-595 (Applied Mechanics and Materials; Vol. 625).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Hamzah, S, Ali, NA, Ariffin, MM & Mohammad, AW 2014, Characterization study of a highly specific affinity membrane for trypsin purification. in Applied Mechanics and Materials. vol. 625, Applied Mechanics and Materials, vol. 625, Trans Tech Publications Ltd, pp. 592-595, 3rd International Conference on Process Engineering and Advanced Materials, ICPEAM 2014, Kuala Lumpur, 3/6/14. https://doi.org/10.4028/www.scientific.net/AMM.625.592
Hamzah S, Ali NA, Ariffin MM, Mohammad AW. Characterization study of a highly specific affinity membrane for trypsin purification. In Applied Mechanics and Materials. Vol. 625. Trans Tech Publications Ltd. 2014. p. 592-595. (Applied Mechanics and Materials). https://doi.org/10.4028/www.scientific.net/AMM.625.592
Hamzah, Sofiah ; Ali, Nora’Aini ; Ariffin, Marinah Mohd ; Mohammad, Abdul Wahab. / Characterization study of a highly specific affinity membrane for trypsin purification. Applied Mechanics and Materials. Vol. 625 Trans Tech Publications Ltd, 2014. pp. 592-595 (Applied Mechanics and Materials).
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