Characterization of the functional domain of β2- microglobulin from the Asian seabass, Lates calcarifer

Hirzahida Mohd-Padil, Khairina Tajul-Arifin, Adura Mohd-Adnan

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    Background: β2-Microglobulin (β2M) is the light chain of major histocompatibility class I (MHC I) that binds non-covalently with the a heavy chain. Both proteins attach to the antigen peptide, presenting a complex to the T cell to be destroyed via the immune mechanism. Methodology/Principal Findings: In this study, a cDNA sequence encoding β2M in the Asian seabass (Lates calcarifer) was identified and analyzed using in silico approaches to predict and characterize its functional domain. The β2M cDNA contains an open reading frame (ORF) of 351 bases with a coding capacity of 116 amino acids. A large portion of the protein consists of the IG constant domain (IGc1), similar to β2M sequences from other species studied thus far. Alignment of the IGc1 domains of β2M from L. calcarifer and other species shows a high degree of overall conservation. Seven amino acids were found to be conserved across taxa whereas conservation between L. calcarifer and other fish species was restricted to 14 amino acids at identical conserved positions. Conclusion/Significance: As the L. calcarifer β2M protein analyzed in this study contains a functional domain similar to that of β2M proteins in other species, it can be postulated that the β2M proteins from L. calcarifer and other organisms are derived from a common ancestor and thus have a similar immune function. Interestingly, fish β2M genes could also be classified according to the ecological habitat of the species, i.e. whether it is from a freshwater, marine or euryhaline environment.

    Original languageEnglish
    Article numbere13159
    JournalPLoS One
    Volume5
    Issue number10
    DOIs
    Publication statusPublished - 2010

    Fingerprint

    Lates calcarifer
    Proteins
    Amino Acids
    Fish
    Conservation
    Fishes
    proteins
    Complementary DNA
    amino acids
    Histocompatibility
    T-cells
    Fresh Water
    Computer Simulation
    Open Reading Frames
    Ecosystem
    fish
    Genes
    open reading frames
    ancestry
    T-Lymphocytes

    ASJC Scopus subject areas

    • Agricultural and Biological Sciences(all)
    • Biochemistry, Genetics and Molecular Biology(all)
    • Medicine(all)

    Cite this

    Characterization of the functional domain of β2- microglobulin from the Asian seabass, Lates calcarifer. / Mohd-Padil, Hirzahida; Tajul-Arifin, Khairina; Mohd-Adnan, Adura.

    In: PLoS One, Vol. 5, No. 10, e13159, 2010.

    Research output: Contribution to journalArticle

    Mohd-Padil, Hirzahida ; Tajul-Arifin, Khairina ; Mohd-Adnan, Adura. / Characterization of the functional domain of β2- microglobulin from the Asian seabass, Lates calcarifer. In: PLoS One. 2010 ; Vol. 5, No. 10.
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