Characterization of T cells and cytokines in the aqueous humour (AH) in patients with Fuchs' heterochromic cyclitis (FHC) and idiopathic anterior uveitis (IAU)

M. Muhaya, V. Calder, H. M A Towler, B. Shaer, M. Mclauchlan, S. Lightman

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

FHC and IAU are two forms of anterior uveitis which are localized to the eyes with no evidence of systemic involvement. However, FHC has distinct clinical features and differs from IAU in that the inflammation is low grade, steroid non-responsive, and has a less aggressive clinical course. To try to dissect the mechanism for this difference the phenotypes of the cells in the AH and blood (PB) and the cytokines present in the AH in patients with FHC and IAU were compared. Three-colour flow cytometry was performed on the cells isolated from the AH and PB. Percentage of cells bearing the following markers were determined: CD3, CD4, CD8, CD4/CD25, CD8/CD25, CD19 and CD14. The cytokines IL-4, IL-10, IL-12 and interferon-gamma (IFN-γ) were assayed by ELISA. In both groups T cell numbers were higher in the AH than PB, although the distribution oft cell subsets in PB was similar. In the AH, CD8+ T cell numbers were higher in FHC than in IAU (P = 0.003), whilst CD4+ numbers were higher in IAU than FHC (P = 0.01). AH cytokine profiles were different in the two groups: IFN-γ levels were higher and IL- 12 levels lower in the FHC group than IAU (P = 0.02), whilst IL-10 levels tended to be higher in the FHC group (P = 0.5). We suggest that different local mechanisms governing the balance of T cell/cytokine-mediated inflammation in the anterior segment may underlie clinical differences such as chronicity and response to steroids in these disorders.

Original languageEnglish
Pages (from-to)123-128
Number of pages6
JournalClinical and Experimental Immunology
Volume111
Issue number1
DOIs
Publication statusPublished - 1998
Externally publishedYes

Fingerprint

Iridocyclitis
Anterior Uveitis
Aqueous Humor
Cytokines
T-Lymphocytes
Interleukin-12
Interleukin-10
Interferon-gamma
Cell Count
Steroids
Inflammation
Interleukin-4
Flow Cytometry
Color
Enzyme-Linked Immunosorbent Assay
Phenotype

Keywords

  • Anterior uveitis
  • Aqueous humour
  • CD8 cytokines
  • Fuchs'

ASJC Scopus subject areas

  • Immunology

Cite this

Characterization of T cells and cytokines in the aqueous humour (AH) in patients with Fuchs' heterochromic cyclitis (FHC) and idiopathic anterior uveitis (IAU). / Muhaya, M.; Calder, V.; Towler, H. M A; Shaer, B.; Mclauchlan, M.; Lightman, S.

In: Clinical and Experimental Immunology, Vol. 111, No. 1, 1998, p. 123-128.

Research output: Contribution to journalArticle

Muhaya, M. ; Calder, V. ; Towler, H. M A ; Shaer, B. ; Mclauchlan, M. ; Lightman, S. / Characterization of T cells and cytokines in the aqueous humour (AH) in patients with Fuchs' heterochromic cyclitis (FHC) and idiopathic anterior uveitis (IAU). In: Clinical and Experimental Immunology. 1998 ; Vol. 111, No. 1. pp. 123-128.
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