Characterization of mononucleated human peripheral blood cells

Ruzanna Ab Kadir, Shahrul Hisham Zainal Ariffin, Rohaya Megat Abdul Wahab, Shabnam Kermani, Sahidan Senafi

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Unspecialized cells that can renew themselves and give rise to multiple differentiated cell types are termed stem cells. The objective of this study was to characterize and investigate, through molecular and biochemical analyses, the stemness of cells derived from isolated mononucleated cells that originated from peripheral blood. The isolated mononucleated cells were separated according to their physical characteristics (adherent and suspension), after 4 to 7 days into a 14-day culturing period in complete medium. Our results revealed that adherent and suspension cells were positive for mesenchymal stem cell (MSC) and hematopoietic stem cell (HSC) markers, respectively. Differentiation of adherent cells into osteoblasts was associated with expression of the OPN gene and increasing ALP enzyme activity, while differentiation of suspension cells into osteoclasts was associated with expression of the TRAP gene and increasing TRAP enzyme activity. In conclusion, molecular and biochemical analyses showed that mononucleated cells consist of MSC (adherent) and HSC (suspension), and both cell types are able to differentiate into specialized cells from their respective lineage: osteoblast (MSC) and osteoclast (HSC).

Original languageEnglish
Article number843843
JournalThe Scientific World Journal
Volume2012
DOIs
Publication statusPublished - 2012

Fingerprint

Stem cells
enzyme activity
Blood Cells
Blood
blood
Cells
gene
Suspensions
stem
Hematopoietic Stem Cells
Mesenchymal Stromal Cells
Osteoblasts
Enzyme activity
Osteoclasts
Genes
Cell Differentiation
Gene Expression
Enzymes
Stem Cells
marker

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Medicine(all)

Cite this

Characterization of mononucleated human peripheral blood cells. / Ab Kadir, Ruzanna; Zainal Ariffin, Shahrul Hisham; Megat Abdul Wahab, Rohaya; Kermani, Shabnam; Senafi, Sahidan.

In: The Scientific World Journal, Vol. 2012, 843843, 2012.

Research output: Contribution to journalArticle

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