Characterization of immunomodulatory activities of honey glycoproteins and glycopeptides

Osman Mesaik Mohammed Ahmed Hassan, Nida Dastagir, Nazim Uddin, Khalid Rehman, M. Kamran Azim

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Recent evidence suggests an important role for natural honey in modulating immune response. To identify active components responsible, this study investigated the immunomodulatory properties of glycoproteins and glycopeptides fractionated from Ziziphus honey. Honey proteins/peptides were fractionated by size exclusion chromatography into five peaks with molecular masses in the range of 2-450 kDa. The fractionated proteins exhibited potent, concentration-dependent inhibition of reactive oxygen species production in zymosan-activated human neutrophils (IC50 = 6-14 ng/mL) and murine macrophages (IC50 = 2-9 ng/mL). Honey proteins significantly suppressed the nitric oxide production by LPS-activated murine macrophages (IC50 = 96-450 ng/mL). Moreover, honey proteins inhibited the phagocytosis latex bead macrophages. The production of pro-inflammatory cytokines IL-1β and TNF-α by human monocytic cell line in the presence of honey proteins was analyzed. Honey proteins did not affect the production of IL-1β; however, TNF-α production was significantly suppressed. These findings indicated that honey glycoproteins and glycopeptides significantly interfere with molecules of the innate immune system.

Original languageEnglish
Pages (from-to)177-184
Number of pages8
JournalJournal of Agricultural and Food Chemistry
Volume63
Issue number1
DOIs
Publication statusPublished - 14 Jan 2015

Fingerprint

glycopeptides
Honey
Glycopeptides
honey
glycoproteins
Glycoproteins
Macrophages
Proteins
Inhibitory Concentration 50
Interleukin-1
inhibitory concentration 50
macrophages
proteins
interleukin-1
Zymosan
Size exclusion chromatography
Immune system
Ziziphus
Latex
Molecular mass

Keywords

  • glycoprotiens
  • honey
  • immunomodulatory
  • oxidative burst
  • phagocytosis

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)
  • Medicine(all)

Cite this

Mohammed Ahmed Hassan, O. M., Dastagir, N., Uddin, N., Rehman, K., & Azim, M. K. (2015). Characterization of immunomodulatory activities of honey glycoproteins and glycopeptides. Journal of Agricultural and Food Chemistry, 63(1), 177-184. https://doi.org/10.1021/jf505131p

Characterization of immunomodulatory activities of honey glycoproteins and glycopeptides. / Mohammed Ahmed Hassan, Osman Mesaik; Dastagir, Nida; Uddin, Nazim; Rehman, Khalid; Azim, M. Kamran.

In: Journal of Agricultural and Food Chemistry, Vol. 63, No. 1, 14.01.2015, p. 177-184.

Research output: Contribution to journalArticle

Mohammed Ahmed Hassan, OM, Dastagir, N, Uddin, N, Rehman, K & Azim, MK 2015, 'Characterization of immunomodulatory activities of honey glycoproteins and glycopeptides', Journal of Agricultural and Food Chemistry, vol. 63, no. 1, pp. 177-184. https://doi.org/10.1021/jf505131p
Mohammed Ahmed Hassan, Osman Mesaik ; Dastagir, Nida ; Uddin, Nazim ; Rehman, Khalid ; Azim, M. Kamran. / Characterization of immunomodulatory activities of honey glycoproteins and glycopeptides. In: Journal of Agricultural and Food Chemistry. 2015 ; Vol. 63, No. 1. pp. 177-184.
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