Characterization of human adipose-derived stem cells and expression of chondrogenic genes during induction of cartilage differentiation

Adila A. Hamid, Ruszymah Idrus, Aminuddin Bin Saim, Somasumdaram Sathappan, Chua Kien Hui

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

OBJECTIVES: Understanding the changes in chondrogenic gene expression that are involved in the differentiation of human adipose-derived stem cells to chondrogenic cells is important prior to using this approach for cartilage repair. The aims of the study were to characterize human adipose-derived stem cells and to examine chondrogenic gene expression after one, two, and three weeks of induction. MATERIALS AND METHODS: Human adipose-derived stem cells at passage 4 were evaluated by flow cytometry to examine the expression of surface markers. These adipose-derived stem cells were tested for adipogenic and osteogenic differentiation capacity. Ribonucleic acid was extracted from the cells for quantitative polymerase chain reaction analysis to determine the expression levels of chondrogenic genes after chondrogenic induction. RESULTS: Human adipose-derived stem cells were strongly positive for the mesenchymal markers CD90, CD73, CD44, CD9, and histocompatibility antigen and successfully differentiated into adipogenic and osteogenic lineages. The human adipose-derived stem cells aggregated and formed a dense matrix after chondrogenic induction. The expression of chondrogenic genes (collagen type II, aggrecan core protein, collagen type XI, COMP, and ELASTIN) was significantly higher after the first week of induction. However, a significantly elevated expression of collagen type X was observed after three weeks of chondrogenic induction. CONCLUSION: Human adipose-derived stem cells retain stem cell characteristics after expansion in culture to passage 4 and serve as a feasible source of cells for cartilage regeneration. Chondrogenesis in human adiposederived stem cells was most prominent after one week of chondrogenic induction.

Original languageEnglish
Pages (from-to)99-106
Number of pages8
JournalClinics
Volume67
Issue number2
DOIs
Publication statusPublished - 2012

Fingerprint

Cartilage
Stem Cells
Gene Expression
CD9 Antigen
Collagen Type XI
Thy-1 Antigens
Collagen Type X
CD44 Antigens
Aggrecans
Chondrogenesis
5'-Nucleotidase
Histocompatibility Antigens
Collagen Type II
Differentiation Antigens
Regeneration
Flow Cytometry
RNA
Polymerase Chain Reaction
Genes

Keywords

  • Adipose
  • Cartilage
  • Cell-based therapy
  • Chondrocytes
  • Chondrogenesis
  • Differentiation
  • Stem cells

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Characterization of human adipose-derived stem cells and expression of chondrogenic genes during induction of cartilage differentiation. / A. Hamid, Adila; Idrus, Ruszymah; Saim, Aminuddin Bin; Sathappan, Somasumdaram; Kien Hui, Chua.

In: Clinics, Vol. 67, No. 2, 2012, p. 99-106.

Research output: Contribution to journalArticle

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