Characterization of clarithromycin resistance in Malaysian isolates of Helicobacter pylori

Norazah Ahmad, Wan Rasinah Zakaria, Sheikh Anwar Abdullah, Ramelah Mohamed

    Research output: Contribution to journalArticle

    29 Citations (Scopus)

    Abstract

    Aim: To characterize the types of mutations present in the 23S rRNA genes of Malaysian isolates of clarithromycin-resistant Helicobacter pylori (H pylori). Methods: Clarithromycin susceptibility of H pylori isolates was determined by E test. Analyses for point mutations in the domain V of 23S rRNA genes in clarithromycin-resistant and -sensitive strains were performed by sequence analysis of amplified polymerase chain reaction products. Restriction fragment length polymorphism was performed using Bsa I and Mbo II enzymes to detect restriction sites that correspond to the mutations in the clarithromycin-resistant strains. Results: Of 187 isolates from 120 patients, four were resistant to clarithromycin, while 183 were sensitive. The MIC of the resistant strains ranged from 1.5 to 24 μg/mL. Two isolates had an A2142G mutation and another two had A2143G mutations. A T2182C mutation was detected in two out of four clarithromycin-resistant isolates and in 13 of 14 clarithromycin-sensitive isolates. Restriction enzyme analyses with Bsa I and Mbo II were able to detect the mutations. Conclusion: Clarithromycin resistance is an uncommon occurrence among Malaysian isolates of H pylori strains and the mutations A2142G and A2143G detected were associated with low-level resistance.

    Original languageEnglish
    Pages (from-to)3161-3165
    Number of pages5
    JournalWorld Journal of Gastroenterology
    Volume15
    Issue number25
    DOIs
    Publication statusPublished - 7 Jul 2009

    Fingerprint

    Clarithromycin
    Helicobacter pylori
    Mutation
    rRNA Genes
    Restriction Mapping
    Point Mutation
    Restriction Fragment Length Polymorphisms
    Sequence Analysis
    Polymerase Chain Reaction
    Enzymes

    Keywords

    • 23S rRNA mutation
    • Clarithromycin resistance
    • Helicobacter pylori
    • Restriction fragment length polymorphism

    ASJC Scopus subject areas

    • Gastroenterology

    Cite this

    Characterization of clarithromycin resistance in Malaysian isolates of Helicobacter pylori. / Ahmad, Norazah; Zakaria, Wan Rasinah; Abdullah, Sheikh Anwar; Mohamed, Ramelah.

    In: World Journal of Gastroenterology, Vol. 15, No. 25, 07.07.2009, p. 3161-3165.

    Research output: Contribution to journalArticle

    Ahmad, Norazah ; Zakaria, Wan Rasinah ; Abdullah, Sheikh Anwar ; Mohamed, Ramelah. / Characterization of clarithromycin resistance in Malaysian isolates of Helicobacter pylori. In: World Journal of Gastroenterology. 2009 ; Vol. 15, No. 25. pp. 3161-3165.
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    abstract = "Aim: To characterize the types of mutations present in the 23S rRNA genes of Malaysian isolates of clarithromycin-resistant Helicobacter pylori (H pylori). Methods: Clarithromycin susceptibility of H pylori isolates was determined by E test. Analyses for point mutations in the domain V of 23S rRNA genes in clarithromycin-resistant and -sensitive strains were performed by sequence analysis of amplified polymerase chain reaction products. Restriction fragment length polymorphism was performed using Bsa I and Mbo II enzymes to detect restriction sites that correspond to the mutations in the clarithromycin-resistant strains. Results: Of 187 isolates from 120 patients, four were resistant to clarithromycin, while 183 were sensitive. The MIC of the resistant strains ranged from 1.5 to 24 μg/mL. Two isolates had an A2142G mutation and another two had A2143G mutations. A T2182C mutation was detected in two out of four clarithromycin-resistant isolates and in 13 of 14 clarithromycin-sensitive isolates. Restriction enzyme analyses with Bsa I and Mbo II were able to detect the mutations. Conclusion: Clarithromycin resistance is an uncommon occurrence among Malaysian isolates of H pylori strains and the mutations A2142G and A2143G detected were associated with low-level resistance.",
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