Characterization of Burkholderia pseudomallei protein BPSL1375 validates the Putative hemolytic activity of the COG3176 N-Acyltransferase family

Laziana Ahmad, Teng Loong Hung, Nor Azurah Mat Akhir, Rahmah Mohamed, Sheila Nathan, Mohd Firdaus Mohd Raih

Research output: Contribution to journalArticle

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Abstract

Background: There are still numerous protein subfamilies within families and superfamilies that do not yet have conclusive empirical experimental evidence providing a specific function. These proteins persist in databases with the annotation of a specific 'putative' function made by association with discernible features in the protein sequence. Results: Here, we report the characterization of one such protein produced by the pathogenic soil bacterium Burkholderia pseudomallei, BPSL1375, which provided evidence for putative hemolysins in the COG3176 family to have experimentally validated hemolytic activity. BPSL1375 can be classified into the N-acyltransferase superfamily, specifically to members of the COG3176 family. Sequence alignments identified seven highly conserved residues (Arg54, Phe58, Asp75, Asp78, Arg99, Glu132 and Arg135), of which several have been implicated with N-acyltransferase activity in previously characterized examples. Using the 3D model of an N-acyltransferase example as a reference, an acyl homoserine lactone synthase, we generated 3D structure models for mutants of six of the seven N-acyltransferase conserved residues (R54, D75, D78, R99, E132 and R135). Both the R99 and R135 mutants resulted in a loss of hemolytic activity while mutations at the other five positions resulted in either reduction or increment in hemolytic activity. Conclusions: The implication of residues previously characterized to be important for N-acyltransferase activity to hemolytic activity for the COG3176 family members of the N-acyltransferase provides validation of the correct placement of the hemolytic capability annotation within the N-acyltransferase superfamily.

Original languageEnglish
Article number270
JournalBMC Microbiology
Volume15
Issue number1
DOIs
Publication statusPublished - 23 Nov 2015

Fingerprint

Burkholderia pseudomallei
Acyltransferases
Proteins
Acyl-Butyrolactones
Hemolysin Proteins
Sequence Alignment
Soil
Databases
Bacteria
Mutation

Keywords

  • BPSL1375
  • Burkholderia pseudomallei
  • Hemolysin
  • N-acyltransferase

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Characterization of Burkholderia pseudomallei protein BPSL1375 validates the Putative hemolytic activity of the COG3176 N-Acyltransferase family. / Ahmad, Laziana; Hung, Teng Loong; Mat Akhir, Nor Azurah; Mohamed, Rahmah; Nathan, Sheila; Mohd Raih, Mohd Firdaus.

In: BMC Microbiology, Vol. 15, No. 1, 270, 23.11.2015.

Research output: Contribution to journalArticle

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AU - Nathan, Sheila

AU - Mohd Raih, Mohd Firdaus

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AB - Background: There are still numerous protein subfamilies within families and superfamilies that do not yet have conclusive empirical experimental evidence providing a specific function. These proteins persist in databases with the annotation of a specific 'putative' function made by association with discernible features in the protein sequence. Results: Here, we report the characterization of one such protein produced by the pathogenic soil bacterium Burkholderia pseudomallei, BPSL1375, which provided evidence for putative hemolysins in the COG3176 family to have experimentally validated hemolytic activity. BPSL1375 can be classified into the N-acyltransferase superfamily, specifically to members of the COG3176 family. Sequence alignments identified seven highly conserved residues (Arg54, Phe58, Asp75, Asp78, Arg99, Glu132 and Arg135), of which several have been implicated with N-acyltransferase activity in previously characterized examples. Using the 3D model of an N-acyltransferase example as a reference, an acyl homoserine lactone synthase, we generated 3D structure models for mutants of six of the seven N-acyltransferase conserved residues (R54, D75, D78, R99, E132 and R135). Both the R99 and R135 mutants resulted in a loss of hemolytic activity while mutations at the other five positions resulted in either reduction or increment in hemolytic activity. Conclusions: The implication of residues previously characterized to be important for N-acyltransferase activity to hemolytic activity for the COG3176 family members of the N-acyltransferase provides validation of the correct placement of the hemolytic capability annotation within the N-acyltransferase superfamily.

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