Characterization of a Group B Streptococcus infection based on the demographics, serotypes, antimicrobial susceptibility and genotypes of selected isolates from sterile and non-sterile isolation sites in three major hospitals in Malaysia

Mohd Emir S Suhaimi, Mohd N. Mohd Desa, Narges Eskandarian, Stella Ganapathy Pillay, Zalina Ismail, Vasantha Kumari Neela, Siti Norbaya Masri, Syafinaz Amin Nordin

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7 Citations (Scopus)

Abstract

Background/purpose: The purpose of this study is to characterize GBS isolates that were collected from three major hospitals in a densely populated area of Klang Valley for their demographics, serotypes, antibiotic susceptibility patterns and genetic background. Methods: Sixty GBS isolates from sterile and non-sterile samples in three major hospitals in the Klang Valley area of Malaysia were collected by convenience sampling from 2012 until March 2014. These isolates were studied for their antimicrobial susceptibilities, serotypes and genotypes. Patients' demographic data and clinical information were collected from lab request forms. Results: Diabetes mellitus was the only underlying condition (7 patients, 23.3%); the remaining samples were from patients who were immunocompromised due to medications. Fifty-nine (98%) isolates were sensitive to penicillin, while 78.3% and 88.3% of the isolates were sensitive to erythromycin and clindamycin, respectively. Serotype Ia was the most common serotype (n = 27, 45%), followed by serotype III (n = 10, 16.7%), V (n = 9, 15%), VI (n = 8, 13.3%), VIII (n = 2, 3.3%) and VII (n = 1, 1.7%). Random Amplified Polymorphic DNA (RAPD) typing showed a diverse genetic pedigree for all isolates, including four major groups that clustered according to geographical location. Conclusion: This preliminary study determines the prevalence of limited common serotypes and antimicrobial resistance in distinct GBS isolates. Nonetheless, the RAPD clustering pattern suggests a close genetic lineage of the GBS isolates based on their isolation sites and location of hospitals.

Original languageEnglish
JournalJournal of Infection and Public Health
DOIs
Publication statusAccepted/In press - 2 Aug 2015

Fingerprint

Streptococcus agalactiae
Malaysia
Genotype
Demography
Infection
DNA Fingerprinting
Clindamycin
Erythromycin
Pedigree
Penicillins
Cluster Analysis
Serogroup
Diabetes Mellitus
Cross-Sectional Studies
Anti-Bacterial Agents
DNA

Keywords

  • Group B Streptococcus
  • Resistant
  • Serotype

ASJC Scopus subject areas

  • Infectious Diseases
  • Public Health, Environmental and Occupational Health

Cite this

Characterization of a Group B Streptococcus infection based on the demographics, serotypes, antimicrobial susceptibility and genotypes of selected isolates from sterile and non-sterile isolation sites in three major hospitals in Malaysia. / Suhaimi, Mohd Emir S; Mohd Desa, Mohd N.; Eskandarian, Narges; Ganapathy Pillay, Stella; Ismail, Zalina; Neela, Vasantha Kumari; Masri, Siti Norbaya; Amin Nordin, Syafinaz.

In: Journal of Infection and Public Health, 02.08.2015.

Research output: Contribution to journalArticle

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abstract = "Background/purpose: The purpose of this study is to characterize GBS isolates that were collected from three major hospitals in a densely populated area of Klang Valley for their demographics, serotypes, antibiotic susceptibility patterns and genetic background. Methods: Sixty GBS isolates from sterile and non-sterile samples in three major hospitals in the Klang Valley area of Malaysia were collected by convenience sampling from 2012 until March 2014. These isolates were studied for their antimicrobial susceptibilities, serotypes and genotypes. Patients' demographic data and clinical information were collected from lab request forms. Results: Diabetes mellitus was the only underlying condition (7 patients, 23.3{\%}); the remaining samples were from patients who were immunocompromised due to medications. Fifty-nine (98{\%}) isolates were sensitive to penicillin, while 78.3{\%} and 88.3{\%} of the isolates were sensitive to erythromycin and clindamycin, respectively. Serotype Ia was the most common serotype (n = 27, 45{\%}), followed by serotype III (n = 10, 16.7{\%}), V (n = 9, 15{\%}), VI (n = 8, 13.3{\%}), VIII (n = 2, 3.3{\%}) and VII (n = 1, 1.7{\%}). Random Amplified Polymorphic DNA (RAPD) typing showed a diverse genetic pedigree for all isolates, including four major groups that clustered according to geographical location. Conclusion: This preliminary study determines the prevalence of limited common serotypes and antimicrobial resistance in distinct GBS isolates. Nonetheless, the RAPD clustering pattern suggests a close genetic lineage of the GBS isolates based on their isolation sites and location of hospitals.",
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AU - Eskandarian, Narges

AU - Ganapathy Pillay, Stella

AU - Ismail, Zalina

AU - Neela, Vasantha Kumari

AU - Masri, Siti Norbaya

AU - Amin Nordin, Syafinaz

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AB - Background/purpose: The purpose of this study is to characterize GBS isolates that were collected from three major hospitals in a densely populated area of Klang Valley for their demographics, serotypes, antibiotic susceptibility patterns and genetic background. Methods: Sixty GBS isolates from sterile and non-sterile samples in three major hospitals in the Klang Valley area of Malaysia were collected by convenience sampling from 2012 until March 2014. These isolates were studied for their antimicrobial susceptibilities, serotypes and genotypes. Patients' demographic data and clinical information were collected from lab request forms. Results: Diabetes mellitus was the only underlying condition (7 patients, 23.3%); the remaining samples were from patients who were immunocompromised due to medications. Fifty-nine (98%) isolates were sensitive to penicillin, while 78.3% and 88.3% of the isolates were sensitive to erythromycin and clindamycin, respectively. Serotype Ia was the most common serotype (n = 27, 45%), followed by serotype III (n = 10, 16.7%), V (n = 9, 15%), VI (n = 8, 13.3%), VIII (n = 2, 3.3%) and VII (n = 1, 1.7%). Random Amplified Polymorphic DNA (RAPD) typing showed a diverse genetic pedigree for all isolates, including four major groups that clustered according to geographical location. Conclusion: This preliminary study determines the prevalence of limited common serotypes and antimicrobial resistance in distinct GBS isolates. Nonetheless, the RAPD clustering pattern suggests a close genetic lineage of the GBS isolates based on their isolation sites and location of hospitals.

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