Characterisation of type 2 and 3 metallothioneins from hevea brasiliensis bark

Azlina Bahari, Keng See Chow, Halimah Alias, Mohd Noor Mat-Isa, Kiew Lian Wan, Nasrul Hadi Shaffe, Arokiaraj Pappusamy, Zainorlina Mohd-Zainuddin, Siti Zakiah Zailani

Research output: Contribution to journalArticle

Abstract

A small collection of ESTs was generated from the bark of the rubber tree clone RRIM 2025. Low molecular weight metal-binding proteins, metallothioneins (MTs), were found to be very abundant in the bark gene expression profile. Multiple sequence alignment and phylogenetic analysis between three Hevea MTs and other plant MTs identified them as type 2 and type 3 proteins. Semi-quantitative PCR analysis indicated that each MT was differentially expressed between bark, leaves and latex, with the lowest transcript abundance in latex. In latex from ethephon-treated trees, all MT isoforms were induced at four hours after treatment while only one of them showed decreased expression after 24 hours. Our findings indicate the existence of a multiple gene family for Hevea MTs and varying roles played by isoforms derived from different rubber tree tissues.

Original languageEnglish
Pages (from-to)257-270
Number of pages14
JournalJournal of Rubber Research
Volume16
Issue number4
Publication statusPublished - Dec 2013

Fingerprint

Hevea brasiliensis
Metallothionein
Latex
latex
Hevea
bark
Rubber
Protein Isoforms
ethephon
sequence alignment
Gene expression
binding proteins
Carrier Proteins
quantitative polymerase chain reaction
Genes
Metals
Molecular weight
metals
Tissue
clones

Keywords

  • Bark
  • DNA sequencing
  • EST
  • Gene expression
  • Hevea brasiliensis
  • Isoform
  • Metallothionein

ASJC Scopus subject areas

  • Plant Science
  • Organic Chemistry

Cite this

Bahari, A., Chow, K. S., Alias, H., Mat-Isa, M. N., Wan, K. L., Shaffe, N. H., ... Zailani, S. Z. (2013). Characterisation of type 2 and 3 metallothioneins from hevea brasiliensis bark. Journal of Rubber Research, 16(4), 257-270.

Characterisation of type 2 and 3 metallothioneins from hevea brasiliensis bark. / Bahari, Azlina; Chow, Keng See; Alias, Halimah; Mat-Isa, Mohd Noor; Wan, Kiew Lian; Shaffe, Nasrul Hadi; Pappusamy, Arokiaraj; Mohd-Zainuddin, Zainorlina; Zailani, Siti Zakiah.

In: Journal of Rubber Research, Vol. 16, No. 4, 12.2013, p. 257-270.

Research output: Contribution to journalArticle

Bahari, A, Chow, KS, Alias, H, Mat-Isa, MN, Wan, KL, Shaffe, NH, Pappusamy, A, Mohd-Zainuddin, Z & Zailani, SZ 2013, 'Characterisation of type 2 and 3 metallothioneins from hevea brasiliensis bark', Journal of Rubber Research, vol. 16, no. 4, pp. 257-270.
Bahari A, Chow KS, Alias H, Mat-Isa MN, Wan KL, Shaffe NH et al. Characterisation of type 2 and 3 metallothioneins from hevea brasiliensis bark. Journal of Rubber Research. 2013 Dec;16(4):257-270.
Bahari, Azlina ; Chow, Keng See ; Alias, Halimah ; Mat-Isa, Mohd Noor ; Wan, Kiew Lian ; Shaffe, Nasrul Hadi ; Pappusamy, Arokiaraj ; Mohd-Zainuddin, Zainorlina ; Zailani, Siti Zakiah. / Characterisation of type 2 and 3 metallothioneins from hevea brasiliensis bark. In: Journal of Rubber Research. 2013 ; Vol. 16, No. 4. pp. 257-270.
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