Characterisation of the barley oxalate oxidase gene and generation of rice transformant

Kin Yip Chee, Pui Ling Tan, Kalaivani K. Nadarajah

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In the last two decades efforts have been made to identify, isolate and characterise disease resistance or defense related genes. One such gene is the oxalate oxidase (OXO; EC 1.2.3.4) gene which is an enzyme that enhances plants disease resistance by catalyzing the conversion of oxalic acid to hydrogen peroxide. Here, we isolated and tmnsfonned a putative OXO gene from a barley BAC clone 450N4 through PCR using specifically designed primers based on the barley OXO cDNA sequence from NCBI. The amplified sequence was identical to the barley OXO gene (Yl 4203) and barley OXO germin protein The amplified gene has a coding region of 675 bp and has been shown to be involved in abiotic and biotic stress regulation The OXO sequence was then introduced into a binary vector that was built using the pGAl 6 and pCambia 301. This gene was then transformed into Oryza saliva indica cultivar IvII{SL The resulting constnict, pCam-Ubi-MyOXO has a maize ubiquitin promoter and a NOS terminator. This constmct was transformed into induced calli from rice immature embryo of a local elite cultivar, 1vfR81, as explant PCR analysis detected the presence of MyOXO gene with transformation efficiency of 1 .7% (5/280). As a defense related gene and the role it plays in biotic stress regulation makes OXO a food candidate for resistance against diseases rice.

Original languageEnglish
Pages (from-to)263-271
Number of pages9
JournalBiotechnology
Volume11
Issue number5
DOIs
Publication statusPublished - 2012

Fingerprint

oxalate oxidase
Hordeum
Genes
Disease Resistance
Plant Diseases
Oryza
Oxalic Acid
Polymerase Chain Reaction
Bony Callus
Ubiquitin
Saliva
Hydrogen Peroxide

Keywords

  • Disease resistance gene
  • Oxalate oxidase
  • Rice transformation
  • Stress regulation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Characterisation of the barley oxalate oxidase gene and generation of rice transformant. / Chee, Kin Yip; Tan, Pui Ling; K. Nadarajah, Kalaivani.

In: Biotechnology, Vol. 11, No. 5, 2012, p. 263-271.

Research output: Contribution to journalArticle

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