Characterisation of T cells and cytokines in the aqueous humour in Fuchs' Heterochromic Cyclitis (FHC) and Idiopathic Anterior Uveitis (IAU)

M. Muhaya, H. M A Towler, B. Shaer, S. Lightman, V. Calder

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Abstract

Purpose: FHC has distinct clinical features and differs from other forms of anterior uveitis in that it is steroid non-responsive. In this study we compared the phenotype of the cells in the anterior chamber and peripheral blood (PB) and the cytokines present in the aqueous humour (AH) in patients with FHC and IAU. Methods: PB and AH were collected from 10 FHC and 18 IAU patients. Three color flow cytometry was performed on the cells isolated from the AH and PB of these patients. The CD3, CD4, CD8, CD4/CD25, CD8/CD25 and CD14 expressions were measured. The cytokines IL-2, IL-4, IL-10, IL-12 and IFN γ were assayed by ELISA. Results: The predominant difference was that more CD8+ T cells were present in the aqueous in FHC than in IAU (p < 0.001).This was associated with an increase in IL-10 and a decrease in IL-12. Conclusion: This study suggests that FHC is characterised by greater numbers of CD8+ T cells in the AH associated with increased IL-10 and reduced IL-12, the key cytokine for the deleterious Th 1 type of response. The dominant IL-10 with its downregulatory effect could explain the low grade chronicity of the disorder and the lack of response to steroids.

Original languageEnglish
JournalInvestigative Ophthalmology and Visual Science
Volume37
Issue number3
Publication statusPublished - 15 Feb 1996
Externally publishedYes

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Iridocyclitis
Anterior Uveitis
Aqueous Humor
Interleukin-10
Cytokines
T-Lymphocytes
Interleukin-12
Steroids
Anterior Chamber
Interleukin-4
Interleukin-2
Flow Cytometry
Color
Enzyme-Linked Immunosorbent Assay
Phenotype

ASJC Scopus subject areas

  • Ophthalmology

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Characterisation of T cells and cytokines in the aqueous humour in Fuchs' Heterochromic Cyclitis (FHC) and Idiopathic Anterior Uveitis (IAU). / Muhaya, M.; Towler, H. M A; Shaer, B.; Lightman, S.; Calder, V.

In: Investigative Ophthalmology and Visual Science, Vol. 37, No. 3, 15.02.1996.

Research output: Contribution to journalArticle

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abstract = "Purpose: FHC has distinct clinical features and differs from other forms of anterior uveitis in that it is steroid non-responsive. In this study we compared the phenotype of the cells in the anterior chamber and peripheral blood (PB) and the cytokines present in the aqueous humour (AH) in patients with FHC and IAU. Methods: PB and AH were collected from 10 FHC and 18 IAU patients. Three color flow cytometry was performed on the cells isolated from the AH and PB of these patients. The CD3, CD4, CD8, CD4/CD25, CD8/CD25 and CD14 expressions were measured. The cytokines IL-2, IL-4, IL-10, IL-12 and IFN γ were assayed by ELISA. Results: The predominant difference was that more CD8+ T cells were present in the aqueous in FHC than in IAU (p < 0.001).This was associated with an increase in IL-10 and a decrease in IL-12. Conclusion: This study suggests that FHC is characterised by greater numbers of CD8+ T cells in the AH associated with increased IL-10 and reduced IL-12, the key cytokine for the deleterious Th 1 type of response. The dominant IL-10 with its downregulatory effect could explain the low grade chronicity of the disorder and the lack of response to steroids.",
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AU - Lightman, S.

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