Characterisation OF Pb-RESISTANT plant growth-promoting rhizobacteria (PGPR) from Scirpus grossus

M. A. Kamaruzzaman, S. R.S. Abdullah, H. A. Hasan, M. Hassan, A. R. Othman, M. Idris

Research output: Contribution to journalArticle

Abstract

Microorganisms that have the potential to improve plant productivity and health are known as plant growth-promoting rhizobacteria. Rhizobacteria play a major role in phytoremediation process by increasing the phytoremediation efficiency, thus the need to identify the superior rhizosphere bacteria has been gaining serious attention. In the current study, Scirpus grossus were exposed to a series of lead-contaminated sand (50, 100, 150, 200 and 300 mg/L) in plastic crates in greenhouse enviroment. Four weeks after exposure, the plants could sustain with toxicity of lead contamination by as much as 300 mg/L. Twenty two colonies of rhizobacteria were isolated from plant roots of Scirpus grossus. The rhizobacteria were characterised based on cell and colony observation. Identification of rhizobacteria was carried out based on analysis of 16S rDNA sequences and were identified as Aeromonas taiwanensis isolate 5E, Bacillus sp. Isolate 7G, Bacillus cereus isolate 8H and 3C, Bacillus velezensis isolate 9I, Bacillus proteolyticus isolate 4D, Bacillus stratosphericus isolate 14N, Bacillus megaterium isolate 11K, Pseudomonas sp. Isolate 12L, Enterobacter cloacae isolate 13M and isolate 16P, Bacillus aerius isolate 15O and Lysinibacillus sp. isolate 10J. The highly lead-resistant rhizobacteria were identified as Bacillus proteolyticus isolate 4D, Bacillus velezensis isolate 9I and Lysinibacillus sp. isolate 10J, which can tolerate against high concentration of lead (300 mg/L). The results showed that these highly lead-resistant rhizobacteria have potential to produce plant growth-promoting traits and have a ability to help S. grossus growth and productivity.

Original languageEnglish
Article number101456
JournalBiocatalysis and Agricultural Biotechnology
Volume23
DOIs
Publication statusPublished - Jan 2020

Fingerprint

Scirpus
plant growth-promoting rhizobacteria
rhizosphere bacteria
Bacillus (bacteria)
Bacilli
Bacillus
Growth
Lead
Lysinibacillus
Environmental Biodegradation
phytoremediation
Bacillus megaterium
Productivity
Aeromonas
Enterobacter cloacae
crates
Rhizosphere
Bacillus cereus
Plant Roots
Ribosomal DNA

Keywords

  • Isolation
  • Lead
  • Phytoremediation
  • Rhizobacteria
  • Scirpus grossus

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Agronomy and Crop Science

Cite this

Characterisation OF Pb-RESISTANT plant growth-promoting rhizobacteria (PGPR) from Scirpus grossus. / Kamaruzzaman, M. A.; Abdullah, S. R.S.; Hasan, H. A.; Hassan, M.; Othman, A. R.; Idris, M.

In: Biocatalysis and Agricultural Biotechnology, Vol. 23, 101456, 01.2020.

Research output: Contribution to journalArticle

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abstract = "Microorganisms that have the potential to improve plant productivity and health are known as plant growth-promoting rhizobacteria. Rhizobacteria play a major role in phytoremediation process by increasing the phytoremediation efficiency, thus the need to identify the superior rhizosphere bacteria has been gaining serious attention. In the current study, Scirpus grossus were exposed to a series of lead-contaminated sand (50, 100, 150, 200 and 300 mg/L) in plastic crates in greenhouse enviroment. Four weeks after exposure, the plants could sustain with toxicity of lead contamination by as much as 300 mg/L. Twenty two colonies of rhizobacteria were isolated from plant roots of Scirpus grossus. The rhizobacteria were characterised based on cell and colony observation. Identification of rhizobacteria was carried out based on analysis of 16S rDNA sequences and were identified as Aeromonas taiwanensis isolate 5E, Bacillus sp. Isolate 7G, Bacillus cereus isolate 8H and 3C, Bacillus velezensis isolate 9I, Bacillus proteolyticus isolate 4D, Bacillus stratosphericus isolate 14N, Bacillus megaterium isolate 11K, Pseudomonas sp. Isolate 12L, Enterobacter cloacae isolate 13M and isolate 16P, Bacillus aerius isolate 15O and Lysinibacillus sp. isolate 10J. The highly lead-resistant rhizobacteria were identified as Bacillus proteolyticus isolate 4D, Bacillus velezensis isolate 9I and Lysinibacillus sp. isolate 10J, which can tolerate against high concentration of lead (300 mg/L). The results showed that these highly lead-resistant rhizobacteria have potential to produce plant growth-promoting traits and have a ability to help S. grossus growth and productivity.",
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