Characterisation of cellulases and xylanase from trichoderma virens UKM1 and its potential in oil palm empty fruit bunch (OPEFB) saccharification

Barbara Ngikoh, Noor Adila Abdul Karim, Jamaliah Md Jahim, Farah Diba Abu Bakar, Abdul Munir Abd. Murad

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A large amount of lignocellulosic waste biomass such as oil palm empty fruit bunch (OPEFB) is produced annually. Enzymes that are responsible for the degradation of lignocellulosic waste play a crucial role in converting the biomass into fermentable sugars and bio products. The aims of this work are to determine the ability of Trichoderma virens UKM1 to produce cellulases and xylanase using OPEFB as substrate in shakeflask fermentation system and to evaluate the potential of these enzymes for OPEFB saccharification. The highest enzyme activities recorded from T. virens UKM1 when 1% OPEFB used as the substrate were 0.79 U/mL for total cellulase (FPase), 53.22 U/mL for endoglucanase (CMCase), 6.5 U/mL for exoglucanase, 0.97 U/mL for β-glucosidase and 254.12 U/mL for xylanase. FPase, CMCase and exoglucanase exhibited their maximum activities at day two incubation, while xylanase and β-glucosidase activities were the highest at day six and seven, respectively. The enzymes obtained from this fungus were then used to hydrolyse OPEFB. The highest glucose production of 1.1 g/L was achieved when 5% OPEFB (w/v) was hydrolysed with 20 FPU enzyme units and with the addition of β-glucosidase (4 U/g OPEFB) for 24 h. These findings suggest that T. virens UKM1 is a potential cellulase producer for saccharification of OPEFB into simple sugars. In addition, OPEFB can be used as a cheap substrate for the production of hydrolytic enzymes from T. virens UKM1.

Original languageEnglish
Pages (from-to)171-184
Number of pages14
JournalJournal of Physical Science
Volume28
DOIs
Publication statusPublished - 2017

Fingerprint

Cellulases
Saccharification
Palm oil
fruits
Fruits
oils
enzymes
Glucosidases
Enzymes
Cellulase
biomass
sugars
Sugars
Biomass
Substrates
enzyme activity
fermentation
fungi
Enzyme activity
Fungi

Keywords

  • Cellulase
  • Fermentable sugars
  • Oil palm empty fruit bunch
  • Saccharification
  • Trichoderma virens

ASJC Scopus subject areas

  • Materials Science(all)
  • Physics and Astronomy(all)

Cite this

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title = "Characterisation of cellulases and xylanase from trichoderma virens UKM1 and its potential in oil palm empty fruit bunch (OPEFB) saccharification",
abstract = "A large amount of lignocellulosic waste biomass such as oil palm empty fruit bunch (OPEFB) is produced annually. Enzymes that are responsible for the degradation of lignocellulosic waste play a crucial role in converting the biomass into fermentable sugars and bio products. The aims of this work are to determine the ability of Trichoderma virens UKM1 to produce cellulases and xylanase using OPEFB as substrate in shakeflask fermentation system and to evaluate the potential of these enzymes for OPEFB saccharification. The highest enzyme activities recorded from T. virens UKM1 when 1{\%} OPEFB used as the substrate were 0.79 U/mL for total cellulase (FPase), 53.22 U/mL for endoglucanase (CMCase), 6.5 U/mL for exoglucanase, 0.97 U/mL for β-glucosidase and 254.12 U/mL for xylanase. FPase, CMCase and exoglucanase exhibited their maximum activities at day two incubation, while xylanase and β-glucosidase activities were the highest at day six and seven, respectively. The enzymes obtained from this fungus were then used to hydrolyse OPEFB. The highest glucose production of 1.1 g/L was achieved when 5{\%} OPEFB (w/v) was hydrolysed with 20 FPU enzyme units and with the addition of β-glucosidase (4 U/g OPEFB) for 24 h. These findings suggest that T. virens UKM1 is a potential cellulase producer for saccharification of OPEFB into simple sugars. In addition, OPEFB can be used as a cheap substrate for the production of hydrolytic enzymes from T. virens UKM1.",
keywords = "Cellulase, Fermentable sugars, Oil palm empty fruit bunch, Saccharification, Trichoderma virens",
author = "Barbara Ngikoh and Karim, {Noor Adila Abdul} and {Md Jahim}, Jamaliah and {Abu Bakar}, {Farah Diba} and {Abd. Murad}, {Abdul Munir}",
year = "2017",
doi = "10.21315/jps2017.28.s1.11",
language = "English",
volume = "28",
pages = "171--184",
journal = "Journal of Physical Science",
issn = "1675-3402",
publisher = "Penerbit Universiti Sains Malaysia",

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T1 - Characterisation of cellulases and xylanase from trichoderma virens UKM1 and its potential in oil palm empty fruit bunch (OPEFB) saccharification

AU - Ngikoh, Barbara

AU - Karim, Noor Adila Abdul

AU - Md Jahim, Jamaliah

AU - Abu Bakar, Farah Diba

AU - Abd. Murad, Abdul Munir

PY - 2017

Y1 - 2017

N2 - A large amount of lignocellulosic waste biomass such as oil palm empty fruit bunch (OPEFB) is produced annually. Enzymes that are responsible for the degradation of lignocellulosic waste play a crucial role in converting the biomass into fermentable sugars and bio products. The aims of this work are to determine the ability of Trichoderma virens UKM1 to produce cellulases and xylanase using OPEFB as substrate in shakeflask fermentation system and to evaluate the potential of these enzymes for OPEFB saccharification. The highest enzyme activities recorded from T. virens UKM1 when 1% OPEFB used as the substrate were 0.79 U/mL for total cellulase (FPase), 53.22 U/mL for endoglucanase (CMCase), 6.5 U/mL for exoglucanase, 0.97 U/mL for β-glucosidase and 254.12 U/mL for xylanase. FPase, CMCase and exoglucanase exhibited their maximum activities at day two incubation, while xylanase and β-glucosidase activities were the highest at day six and seven, respectively. The enzymes obtained from this fungus were then used to hydrolyse OPEFB. The highest glucose production of 1.1 g/L was achieved when 5% OPEFB (w/v) was hydrolysed with 20 FPU enzyme units and with the addition of β-glucosidase (4 U/g OPEFB) for 24 h. These findings suggest that T. virens UKM1 is a potential cellulase producer for saccharification of OPEFB into simple sugars. In addition, OPEFB can be used as a cheap substrate for the production of hydrolytic enzymes from T. virens UKM1.

AB - A large amount of lignocellulosic waste biomass such as oil palm empty fruit bunch (OPEFB) is produced annually. Enzymes that are responsible for the degradation of lignocellulosic waste play a crucial role in converting the biomass into fermentable sugars and bio products. The aims of this work are to determine the ability of Trichoderma virens UKM1 to produce cellulases and xylanase using OPEFB as substrate in shakeflask fermentation system and to evaluate the potential of these enzymes for OPEFB saccharification. The highest enzyme activities recorded from T. virens UKM1 when 1% OPEFB used as the substrate were 0.79 U/mL for total cellulase (FPase), 53.22 U/mL for endoglucanase (CMCase), 6.5 U/mL for exoglucanase, 0.97 U/mL for β-glucosidase and 254.12 U/mL for xylanase. FPase, CMCase and exoglucanase exhibited their maximum activities at day two incubation, while xylanase and β-glucosidase activities were the highest at day six and seven, respectively. The enzymes obtained from this fungus were then used to hydrolyse OPEFB. The highest glucose production of 1.1 g/L was achieved when 5% OPEFB (w/v) was hydrolysed with 20 FPU enzyme units and with the addition of β-glucosidase (4 U/g OPEFB) for 24 h. These findings suggest that T. virens UKM1 is a potential cellulase producer for saccharification of OPEFB into simple sugars. In addition, OPEFB can be used as a cheap substrate for the production of hydrolytic enzymes from T. virens UKM1.

KW - Cellulase

KW - Fermentable sugars

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KW - Saccharification

KW - Trichoderma virens

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