Characterisation of a human bilirubin UDP-glucuronosyltransferase stably expressed in hamster lung fibroblast cell cultures

Lesley Sutherland, Sahidan Senafi, Thomas Ebner, Douglas J. Clarke, Brian Burchell

Research output: Contribution to journal › Article

31 Citations (Scopus)

Abstract

A cDNA encoding a human bilirubin UDP-glucuronosyltransferase has been isolated and stably expressed in Chinese hamster V79 lung fibroblast cell line. Western blotting of cell homogenates with anti-UGT antibody revealed a highly expressed protein of approx. 55.5 kDa in size. The expressed enzyme specifically catalysed the formation of bilirubin mono- and diglucuronides, and also catalysed the glucuronidation of two phenolic compounds, which are good substrates for other human UGT isoenzymes, at low rates.

Original languageEnglish
Pages (from-to)161-164
Number of pages4
JournalFEBS Letters
Volume308
Issue number2
DOIs
Publication statusPublished - 17 Aug 1992
Externally publishedYes

Fingerprint

bilirubin glucuronoside glucuronosyltransferase
Fibroblasts
Cell culture
Cricetinae
Isoenzymes
Complementary DNA
Cell Culture Techniques
Cells
Lung
Antibodies
Substrates
Enzymes
Cricetulus
Anti-Idiotypic Antibodies
Proteins
Western Blotting
Cell Line
bilirubin glucuronate
bilirubin diglucuronide

Keywords

  • Complementary DNA
  • Enzyme activity
  • Eukaryotic expression vector

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Characterisation of a human bilirubin UDP-glucuronosyltransferase stably expressed in hamster lung fibroblast cell cultures. / Sutherland, Lesley; Senafi, Sahidan; Ebner, Thomas; Clarke, Douglas J.; Burchell, Brian.

In: FEBS Letters, Vol. 308, No. 2, 17.08.1992, p. 161-164.

Research output: Contribution to journal › Article

Sutherland, L, Senafi, S, Ebner, T, Clarke, DJ & Burchell, B 1992, 'Characterisation of a human bilirubin UDP-glucuronosyltransferase stably expressed in hamster lung fibroblast cell cultures', FEBS Letters, vol. 308, no. 2, pp. 161-164. https://doi.org/10.1016/0014-5793(92)81266-O
Sutherland L, Senafi S, Ebner T, Clarke DJ, Burchell B. Characterisation of a human bilirubin UDP-glucuronosyltransferase stably expressed in hamster lung fibroblast cell cultures. FEBS Letters. 1992 Aug 17;308(2):161-164. https://doi.org/10.1016/0014-5793(92)81266-O
Sutherland, Lesley ; Senafi, Sahidan ; Ebner, Thomas ; Clarke, Douglas J. ; Burchell, Brian. / Characterisation of a human bilirubin UDP-glucuronosyltransferase stably expressed in hamster lung fibroblast cell cultures. In: FEBS Letters. 1992 ; Vol. 308, No. 2. pp. 161-164.
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