Abstract
A cDNA encoding a human bilirubin UDP-glucuronosyltransferase has been isolated and stably expressed in Chinese hamster V79 lung fibroblast cell line. Western blotting of cell homogenates with anti-UGT antibody revealed a highly expressed protein of approx. 55.5 kDa in size. The expressed enzyme specifically catalysed the formation of bilirubin mono- and diglucuronides, and also catalysed the glucuronidation of two phenolic compounds, which are good substrates for other human UGT isoenzymes, at low rates.
Original language | English |
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Pages (from-to) | 161-164 |
Number of pages | 4 |
Journal | FEBS Letters |
Volume | 308 |
Issue number | 2 |
DOIs | |
Publication status | Published - 17 Aug 1992 |
Externally published | Yes |
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Keywords
- Complementary DNA
- Enzyme activity
- Eukaryotic expression vector
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Characterisation of a human bilirubin UDP-glucuronosyltransferase stably expressed in hamster lung fibroblast cell cultures. / Sutherland, Lesley; Senafi, Sahidan; Ebner, Thomas; Clarke, Douglas J.; Burchell, Brian.
In: FEBS Letters, Vol. 308, No. 2, 17.08.1992, p. 161-164.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Characterisation of a human bilirubin UDP-glucuronosyltransferase stably expressed in hamster lung fibroblast cell cultures
AU - Sutherland, Lesley
AU - Senafi, Sahidan
AU - Ebner, Thomas
AU - Clarke, Douglas J.
AU - Burchell, Brian
PY - 1992/8/17
Y1 - 1992/8/17
N2 - A cDNA encoding a human bilirubin UDP-glucuronosyltransferase has been isolated and stably expressed in Chinese hamster V79 lung fibroblast cell line. Western blotting of cell homogenates with anti-UGT antibody revealed a highly expressed protein of approx. 55.5 kDa in size. The expressed enzyme specifically catalysed the formation of bilirubin mono- and diglucuronides, and also catalysed the glucuronidation of two phenolic compounds, which are good substrates for other human UGT isoenzymes, at low rates.
AB - A cDNA encoding a human bilirubin UDP-glucuronosyltransferase has been isolated and stably expressed in Chinese hamster V79 lung fibroblast cell line. Western blotting of cell homogenates with anti-UGT antibody revealed a highly expressed protein of approx. 55.5 kDa in size. The expressed enzyme specifically catalysed the formation of bilirubin mono- and diglucuronides, and also catalysed the glucuronidation of two phenolic compounds, which are good substrates for other human UGT isoenzymes, at low rates.
KW - Complementary DNA
KW - Enzyme activity
KW - Eukaryotic expression vector
UR - http://www.scopus.com/inward/record.url?scp=0026625085&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026625085&partnerID=8YFLogxK
U2 - 10.1016/0014-5793(92)81266-O
DO - 10.1016/0014-5793(92)81266-O
M3 - Article
C2 - 1499725
AN - SCOPUS:0026625085
VL - 308
SP - 161
EP - 164
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 2
ER -