Characterisation and stability of pigments extracted from Sargassum binderi obtained from Semporna, Sabah

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8 Citations (Scopus)

Abstract

This study was carried out to identify the pigment extracted from Malaysian brown seaweed, Sargassum binderi and its stability in various conditions. Pigments were extracted using methanol:chloroform:water (4:2:1, v/v/v), which is part of fucoidan extraction process, where the pigments were waste. Carotenoid and chlorophyll were found in the extract using UV-vis spectrophotometer (420 and 672 nm, respectively). Fucoxanthin was identified as the carotenoid present using HPLC, while its functional groups and structure were determined using FTIR and1H NMR, respectively. The fucoxanthin-rich extract stability was tested on different pH (pH1-13), light exposure (dark and light) and storage temperature (4°C, 25°C and 50°C). The stability tests showed that it was most stable at pH5-7, stored in dark condition and at the storage temperature of 4°C and 25°C. The fucoxanthin-rich extract from Sargassum binderi has potential to be applied as bioingredient and functional food as it is stable in normal storage conditions.

Original languageEnglish
Pages (from-to)1345-1354
Number of pages10
JournalSains Malaysiana
Volume43
Issue number9
Publication statusPublished - 1 Sep 2014

Fingerprint

Sargassum
Borneo
pigments
storage temperature
extracts
carotenoids
fucoidan
Phaeophyceae
spectrophotometers
storage conditions
chloroform
functional foods
methanol
chlorophyll
water
testing

Keywords

  • Carotenoid
  • Chlorophyll
  • Fucoxanthin
  • Pigment
  • Sargassum binderi

ASJC Scopus subject areas

  • General

Cite this

Characterisation and stability of pigments extracted from Sargassum binderi obtained from Semporna, Sabah. / Yip, Wu Hon; Lim, Seng Joe; Wan Mustapha, Wan Aida; Maskat, Mohamad Yusof; Said, Mamot.

In: Sains Malaysiana, Vol. 43, No. 9, 01.09.2014, p. 1345-1354.

Research output: Contribution to journalArticle

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