Chaperonin CCT checkpoint function in basal transcription factor TFIID assembly

Simona V. Antonova, Matthias Haffke, Eleonora Corradini, Mykolas Mikuciunas, Low Teck Yew, Luca Signor, Robert M. van Es, Kapil Gupta, Elisabeth Scheer, Harmjan R. Vos, László Tora, Albert J.R. Heck, H. T.Marc Timmers, Imre Berger

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

TFIID is a cornerstone of eukaryotic gene regulation. Distinct TFIID complexes with unique subunit compositions exist and several TFIID subunits are shared with other complexes, thereby conveying precise cellular control of subunit allocation and functional assembly of this essential transcription factor. However, the molecular mechanisms that underlie the regulation of TFIID remain poorly understood. Here we use quantitative proteomics to examine TFIID submodules and assembly mechanisms in human cells. Structural and mutational analysis of the cytoplasmic TAF5–TAF6–TAF9 submodule identified novel interactions that are crucial for TFIID integrity and for allocation of TAF9 to TFIID or the Spt-Ada-Gcn5 acetyltransferase (SAGA) co-activator complex. We discover a key checkpoint function for the chaperonin CCT, which specifically associates with nascent TAF5 for subsequent handover to TAF6–TAF9 and ultimate holo-TFIID formation. Our findings illustrate at the molecular level how multisubunit complexes are generated within the cell via mechanisms that involve checkpoint decisions facilitated by a chaperone.

Original languageEnglish
Pages (from-to)1119-1127
Number of pages9
JournalNature Structural and Molecular Biology
Volume25
Issue number12
DOIs
Publication statusPublished - 1 Dec 2018

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Chaperonin Containing TCP-1
Transcription Factor TFIID
Acetyltransferases
Proteomics
Transcription Factors

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

Cite this

Antonova, S. V., Haffke, M., Corradini, E., Mikuciunas, M., Teck Yew, L., Signor, L., ... Berger, I. (2018). Chaperonin CCT checkpoint function in basal transcription factor TFIID assembly. Nature Structural and Molecular Biology, 25(12), 1119-1127. https://doi.org/10.1038/s41594-018-0156-z

Chaperonin CCT checkpoint function in basal transcription factor TFIID assembly. / Antonova, Simona V.; Haffke, Matthias; Corradini, Eleonora; Mikuciunas, Mykolas; Teck Yew, Low; Signor, Luca; van Es, Robert M.; Gupta, Kapil; Scheer, Elisabeth; Vos, Harmjan R.; Tora, László; Heck, Albert J.R.; Timmers, H. T.Marc; Berger, Imre.

In: Nature Structural and Molecular Biology, Vol. 25, No. 12, 01.12.2018, p. 1119-1127.

Research output: Contribution to journalArticle

Antonova, SV, Haffke, M, Corradini, E, Mikuciunas, M, Teck Yew, L, Signor, L, van Es, RM, Gupta, K, Scheer, E, Vos, HR, Tora, L, Heck, AJR, Timmers, HTM & Berger, I 2018, 'Chaperonin CCT checkpoint function in basal transcription factor TFIID assembly', Nature Structural and Molecular Biology, vol. 25, no. 12, pp. 1119-1127. https://doi.org/10.1038/s41594-018-0156-z
Antonova, Simona V. ; Haffke, Matthias ; Corradini, Eleonora ; Mikuciunas, Mykolas ; Teck Yew, Low ; Signor, Luca ; van Es, Robert M. ; Gupta, Kapil ; Scheer, Elisabeth ; Vos, Harmjan R. ; Tora, László ; Heck, Albert J.R. ; Timmers, H. T.Marc ; Berger, Imre. / Chaperonin CCT checkpoint function in basal transcription factor TFIID assembly. In: Nature Structural and Molecular Biology. 2018 ; Vol. 25, No. 12. pp. 1119-1127.
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