Changes in oxidative stress in transgenic RNAi ACO1 tomato fruit during ripening

Najat Mohamed Eglous, Zainon Mohd Ali, Maizom Hassan, Zamri Zainal

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Tomato (Solanum Lycopersicum L.) is the second most cultivated vegetable in the world and widely used as a system for studying the role of ethylene during fruit ripening. Our objective was to study the oxidative stress and antioxidative metabolism during ripening of non transgenic tomato and transgenic line-21 tomato which reduced ethylene. The line-21 of transgenic tomato plants (RNAi ACO1) had lower ethylene production and longer shelf-life more than 32 days as compared to the wild-type fruits which have very short shelf-life. In this study, tomato fruit were divided into five different stages (MG: mature green 5%, B: breaker 25%, T: turning 50%, O: orange75%, RR: red ripe100%).The activity of lipoxygenase (LOX) and lipid peroxidation (MDA) were measured to assess changes in oxidative stress. The LOX activity and MDA content decreased significantly obtaining 2.6-fold and 1.2-fold, respectively, as compared to the wild type fruit. However, superoxide dismutase (SOD) and catalase (CAT) activities were increased to 1.9 and 1.2 folds from the mature green to the fully ripe stage in transgenic tomatoes. Furthermore, the wild type tomato increases 1.3 in SOD and 1.6 in CAT activities. The overall results indicate that the wild type tomato fruit showed a faster rate of ripening, parallel to decline in the rate of enzymatic antioxidative systems as compared to the transgenic line-21 tomato fruit. In addition, the results show that the antioxidant capacity is improved during the ripening process and is accompanied by an increase in the oxidative stress.

Original languageEnglish
Title of host publicationAIP Conference Proceedings
Pages215-221
Number of pages7
Volume1571
DOIs
Publication statusPublished - 2013
Event2013 UKM Faculty of Science and Technology Post-Graduate Colloquium - Selangor
Duration: 3 Jul 20134 Jul 2013

Other

Other2013 UKM Faculty of Science and Technology Post-Graduate Colloquium
CitySelangor
Period3/7/134/7/13

Fingerprint

tomatoes
fruits
multiple docking adapters
catalase
ethylene
inorganic peroxides
life (durability)
circuit breakers
vegetables
antioxidants
metabolism
lipids

Keywords

  • Antioxidant enzymes
  • Oxidative processes
  • Ripening

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Changes in oxidative stress in transgenic RNAi ACO1 tomato fruit during ripening. / Eglous, Najat Mohamed; Ali, Zainon Mohd; Hassan, Maizom; Zainal, Zamri.

AIP Conference Proceedings. Vol. 1571 2013. p. 215-221.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Eglous, NM, Ali, ZM, Hassan, M & Zainal, Z 2013, Changes in oxidative stress in transgenic RNAi ACO1 tomato fruit during ripening. in AIP Conference Proceedings. vol. 1571, pp. 215-221, 2013 UKM Faculty of Science and Technology Post-Graduate Colloquium, Selangor, 3/7/13. https://doi.org/10.1063/1.4858657
Eglous, Najat Mohamed ; Ali, Zainon Mohd ; Hassan, Maizom ; Zainal, Zamri. / Changes in oxidative stress in transgenic RNAi ACO1 tomato fruit during ripening. AIP Conference Proceedings. Vol. 1571 2013. pp. 215-221
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