Changes in hepatic phosphoprotein levels in mice infected with plasmodium berghei

Pramila Maniam, Zainal Abidin Abu Hassan, Mohammed Noor Embi, Hasidah Mohd. Sidek

Research output: Contribution to journalArticle

Abstract

Hepatic phosphoprotein levels are altered in mouse liver as a manifestation of bacteria, virus or parasite infection. Identification of signaling pathways mediated by these hepatic proteins contribute to the current understanding of the mechanism of pathogenesis in malarial infection. The present study was undertaken to evaluate the changes in hepatic phosphoprotein levels during Plasmodium berghei infection. Our study revealed changes in levels of three hepatic phosphoproteins following P. berghei infection compared to non-infected controls. Peptide fragment sequence analysis using tandem mass spectrometry (MS/MS) showed these hepatic proteins to be homologs to haemoglobin beta (HBB), class Pi glutathione S-tranferase (GSTPi) and carbonic anhydrase III (CAIII) proteins of Mus musculus species respectively from the NCBInr sequence database. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis predicted the involvement of these proteins in specific pathways in Mus musculus species; GSTPi in glutathione and drug metabolism and CAIII in nitrogen metabolism. This shows that P. berghei infection affects similar signaling pathways as those reported in other pathogenic infections such as that related to GSTPi and CAIII in response to oxidative stress. Copyright Reserved

Original languageEnglish
Pages (from-to)721-729
Number of pages9
JournalSains Malaysiana
Volume41
Issue number6
Publication statusPublished - Jun 2012

Fingerprint

Plasmodium berghei
Phosphoproteins
Carbonic Anhydrase III
Liver
Malaria
Glutathione
Proteins
Encyclopedias
Parasitic Diseases
Peptide Fragments
Protein Sequence Analysis
Virus Diseases
Tandem Mass Spectrometry
Infection
Hemoglobins
Oxidative Stress
Nitrogen
Genome
Databases
Bacteria

Keywords

  • Chloroquine
  • Hepatomegaly
  • Malaria
  • Phosphoprotein
  • Plasmodium berghei

ASJC Scopus subject areas

  • General

Cite this

Changes in hepatic phosphoprotein levels in mice infected with plasmodium berghei. / Maniam, Pramila; Hassan, Zainal Abidin Abu; Embi, Mohammed Noor; Mohd. Sidek, Hasidah.

In: Sains Malaysiana, Vol. 41, No. 6, 06.2012, p. 721-729.

Research output: Contribution to journalArticle

Maniam, Pramila ; Hassan, Zainal Abidin Abu ; Embi, Mohammed Noor ; Mohd. Sidek, Hasidah. / Changes in hepatic phosphoprotein levels in mice infected with plasmodium berghei. In: Sains Malaysiana. 2012 ; Vol. 41, No. 6. pp. 721-729.
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