Challenges in culturing Macaca fascicularis bone Marrow stem cells

S. Sharensharen Aini, Masfueh Razali, Badiah Baharin, Min Hwei Ng, B. S. Shamsul, Chua Kien Hui, C. K. Low, Mohd Nazimi Abd Jabar, Rifqah Nordin, Norziha Yahaya, A. S. Shariffah Shuriana, Ruszymah Idrus, C. Siar, Roszalina Ramli

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Culturing Macaca fascicularis bone marrow stem cells in fetal bovine serum (FBS) resulted in low proliferation and long period of incubation. Therefore, its potential uses are exhausted. Here we report the establishment of culturing the Macaca fascicularis bone marrow stem cells using the FBS in combination with autologous serum. Five percent autologous serum was added to the Minimum Essential Medium (MEM) alpha medium and 10% FBS while 0.2 mM acid ascorbic 2-phosphate, 10 mM β-glycerolphosphate, 10-8 molar dexamethasone were used for osteogenic induction. Following this combination, our results showed higher growth kinetic i.e. 1.41% growth rate higher compared to only 0.46% growth rates of the cells using FBS alone and shorter population doubling time (4 to 7 days) compared to the culture without the combination of FBS and autologous serum (30 days). Thus, the combination of the FBS and autologous serum permits fast cell growth and tissue construction.

Original languageEnglish
Pages (from-to)419-422
Number of pages4
JournalSains Malaysiana
Volume41
Issue number4
Publication statusPublished - Apr 2012

Fingerprint

Macaca fascicularis
Bone Marrow Cells
Stem Cells
Serum
Growth
Glycerophosphates
Dexamethasone

Keywords

  • Bone marrow stem cells
  • Macaca fascicularis
  • Tissue engineering

ASJC Scopus subject areas

  • General

Cite this

Challenges in culturing Macaca fascicularis bone Marrow stem cells. / Sharensharen Aini, S.; Razali, Masfueh; Baharin, Badiah; Ng, Min Hwei; Shamsul, B. S.; Kien Hui, Chua; Low, C. K.; Abd Jabar, Mohd Nazimi; Nordin, Rifqah; Yahaya, Norziha; Shariffah Shuriana, A. S.; Idrus, Ruszymah; Siar, C.; Ramli, Roszalina.

In: Sains Malaysiana, Vol. 41, No. 4, 04.2012, p. 419-422.

Research output: Contribution to journalArticle

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AU - Razali, Masfueh

AU - Baharin, Badiah

AU - Ng, Min Hwei

AU - Shamsul, B. S.

AU - Kien Hui, Chua

AU - Low, C. K.

AU - Abd Jabar, Mohd Nazimi

AU - Nordin, Rifqah

AU - Yahaya, Norziha

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AU - Idrus, Ruszymah

AU - Siar, C.

AU - Ramli, Roszalina

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