Cgl-SLT2 is required for appressorium formation, sporulation and pathogenicity in Colletotrichum gloeosporioides

H. Y. Yong, Farah Diba Abu Bakar, R. M. Illias, N. M. Mahadi, Abdul Munir Abd. Murad

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The mitogen-activated protein (MAP) kinase pathways has been implicated in the pathogenicity of various pathogenic fungi and plays important roles in regulating pathogenicity-related morphogenesis. This work describes the isolation and characterization of MAP kinase gene, Cgl-SLT2, from Colletotrichum gloeosporioides. A DNA sequence, including 1,633 bp of Cgl-SLT2 open-reading frame and its promoter and terminator regions, was isolated viaDNAwalking and cloned. To analyze gene function, a gene disruption cassette containing hygromycin-resistant gene was constructed, and Cgl-SLT2 was inactivated via gene deletion. Analysis on Cgl-slt2 mutant revealed a defect in vegetative growth and sporulation as compared to the wild-type strain. When grown under nutrient-limiting conditions, hyperbranched hyphal morphology was observed in the mutant. Conidia induction for germination on rubber wax-coated hard surfaces revealed no differences in the percentage of conidial germination between the wild-type and Cgl-slt2 mutant. However, the percentage of appressorium formation in the mutant was greatly reduced. Bipolar germination in the mutant was higher than in the wild-type at 8-h post-induction. A pathogenicity assay revealed that the mutant was unable to infect either wounded or unwounded mangoes. These results suggest that the Cgl-SLT2 MAP kinase is required for C. gloeosporioides conidiation, polarized growth, appressorium formation and pathogenicity.

Original languageEnglish
Pages (from-to)1241-1250
Number of pages10
JournalBrazilian Journal of Microbiology
Volume44
Issue number4
DOIs
Publication statusPublished - 2013

Fingerprint

Colletotrichum
Virulence
Germination
Mitogen-Activated Protein Kinases
Genes
Genetic Terminator Regions
Mangifera
Fungal Spores
Waxes
Rubber
Gene Deletion
Growth
Morphogenesis
Genetic Promoter Regions
Open Reading Frames
Fungi
Food

Keywords

  • Appressorium
  • Colletotrichum gloeosporioides
  • MAP kinase
  • Pathogenicity

ASJC Scopus subject areas

  • Microbiology
  • Medicine(all)

Cite this

Cgl-SLT2 is required for appressorium formation, sporulation and pathogenicity in Colletotrichum gloeosporioides. / Yong, H. Y.; Abu Bakar, Farah Diba; Illias, R. M.; Mahadi, N. M.; Abd. Murad, Abdul Munir.

In: Brazilian Journal of Microbiology, Vol. 44, No. 4, 2013, p. 1241-1250.

Research output: Contribution to journalArticle

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