Centella asiatica modulates neuron cell survival by altering caspase-9 pathway

Norfaizatul Shalida Omar, Zetty Akmal Che Zakaria, Then Sue Mian, Wan Zurinah Wan Ngah, Musalmah Mazlan

    Research output: Contribution to journalArticle

    6 Citations (Scopus)

    Abstract

    Natural products have been reported to exert positive impact on neurodegenerative diseases which arise as a result of neuronal loss associated with oxidative stress. Therefore, this study was conducted to evaluate the neuroprotective potential of ethanolic extract of Centella asiatica (CA) compared to that of tocotrienol rich fraction (TRF) using human neuroblastoma, SH-SY5Y cells. Cytotoxicity and neuroprotective effects of CA and TRF were measured by using 3-(4, 5-dimethylthiazol-2-yl)-5-(3- carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt (MTS) against BSO-induced neuron cell death. The rate of apoptotic cell death was measured via FITC Annexin V apoptosis flow cytometer. Presence of live and apoptotic cells were further confirmed with calcein-AM and propidium iodide fluorescence staining. Caspase-8 and-9 were measured to determine the mechanism of activation of the apoptotic pathway. Results showed that CA extract was toxic to neuron cell culture at ≥100 μg/ml. TRF (1 to 50 μg/ml), as positive control and CA (1 to 50 μg/ml) conferred significant protection against BSO-induced cell death. Post-treatment with CA also significantly reduced caspase-9 activity againstBSO-induced cell death. In conclusion, this study shows that low concentration of ethanolic extract of CA is able to protect neuron cells from oxidative stress probably by inhibiting the activation of caspase- 9 pathway but it can exert neurocytotoxic effect at high concentrations.

    Original languageEnglish
    Pages (from-to)2201-2209
    Number of pages9
    JournalJournal of Medicinal Plants Research
    Volume5
    Issue number11
    Publication statusPublished - 4 Jun 2011

    Fingerprint

    Centella
    Centella asiatica
    caspase-9
    Caspase 9
    Tocotrienols
    cell viability
    Cell Survival
    Cell Death
    neurons
    Neurons
    tocotrienols
    cell death
    Oxidative Stress
    Tetrazolium Salts
    Caspase 8
    Propidium
    Fluorescein-5-isothiocyanate
    Poisons
    Annexin A5
    extracts

    Keywords

    • Antioxidant
    • Apoptosis
    • Caspase
    • Centella asiatica l.
    • Neuroprotection

    ASJC Scopus subject areas

    • Pharmacology
    • Complementary and alternative medicine
    • Drug Discovery
    • Plant Science
    • Pharmaceutical Science

    Cite this

    Omar, N. S., Zakaria, Z. A. C., Mian, T. S., Ngah, W. Z. W., & Mazlan, M. (2011). Centella asiatica modulates neuron cell survival by altering caspase-9 pathway. Journal of Medicinal Plants Research, 5(11), 2201-2209.

    Centella asiatica modulates neuron cell survival by altering caspase-9 pathway. / Omar, Norfaizatul Shalida; Zakaria, Zetty Akmal Che; Mian, Then Sue; Ngah, Wan Zurinah Wan; Mazlan, Musalmah.

    In: Journal of Medicinal Plants Research, Vol. 5, No. 11, 04.06.2011, p. 2201-2209.

    Research output: Contribution to journalArticle

    Omar, NS, Zakaria, ZAC, Mian, TS, Ngah, WZW & Mazlan, M 2011, 'Centella asiatica modulates neuron cell survival by altering caspase-9 pathway', Journal of Medicinal Plants Research, vol. 5, no. 11, pp. 2201-2209.
    Omar NS, Zakaria ZAC, Mian TS, Ngah WZW, Mazlan M. Centella asiatica modulates neuron cell survival by altering caspase-9 pathway. Journal of Medicinal Plants Research. 2011 Jun 4;5(11):2201-2209.
    Omar, Norfaizatul Shalida ; Zakaria, Zetty Akmal Che ; Mian, Then Sue ; Ngah, Wan Zurinah Wan ; Mazlan, Musalmah. / Centella asiatica modulates neuron cell survival by altering caspase-9 pathway. In: Journal of Medicinal Plants Research. 2011 ; Vol. 5, No. 11. pp. 2201-2209.
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