Cellular and molecular interactions of Magnaporthe oryzae S3 in rice

Kalaivani K. Nadarajah, Hari Kumar Krishnan, Hamdia Z. Ali

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The interaction between Magnaporthe oryzae S3 and rice was elucidated at the cellular and molecular level. The interactions at the cellular level was observed under SEM where the level of inter and intracellular proliferation of the microbe in three rice varieties was tested yielding Pongsu Seribu as the most resistant. Through DAB assays we detected reactive oxygen species (ROIs) being expressed at the location of pathogen proliferation. In Pongsu Seribu the location of proliferation and the neighboring cells showed elicitation of ROIs. As ROIs are known to trigger defense response, the expression of a defense gene, Pathogenesis related gene(PJ?-lb), was quantified in rice varieties in response to infection. Pongsu Seribu showed the highest level of PR-lb gene expression that is in line with the variety being the most resistant.

Original languageEnglish
Pages (from-to)3481-3491
Number of pages11
JournalJournal of Pure and Applied Microbiology
Volume8
Issue number5
Publication statusPublished - 1 Oct 2014

Fingerprint

Magnaporthe
Genes
Reactive Oxygen Species
Cell Proliferation
Gene Expression
Oryza
Infection

Keywords

  • DAB staining
  • Magnaporthe oryzae
  • Pathogenesis related protein
  • ROI

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Biotechnology
  • Microbiology

Cite this

Cellular and molecular interactions of Magnaporthe oryzae S3 in rice. / K. Nadarajah, Kalaivani; Krishnan, Hari Kumar; Ali, Hamdia Z.

In: Journal of Pure and Applied Microbiology, Vol. 8, No. 5, 01.10.2014, p. 3481-3491.

Research output: Contribution to journalArticle

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