Cellular and Molecular Interaction of Human Dermal Fibroblasts with Bacterial Nanocellulose Composite Hydrogel for Tissue Regeneration

Evelyn Yun Xi Loh, Mh Busra Fauzi, Min Hwei Ng, Pei Yuen Ng, Shiow Fern Ng, Hidayah Ariffin, Mohd Cairul Iqbal Mohd Amin

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The evaluation of the interaction of cells with biomaterials is fundamental to establish the suitability of the biomaterial for a specific application. In this study, the properties of bacterial nanocellulose/acrylic acid (BNC/AA) hydrogels fabricated with varying BNC to AA ratios and electron-beam irradiation doses were determined. The manner these hydrogel properties influence the behavior of human dermal fibroblasts (HDFs) at the cellular and molecular levels was also investigated, relating it to its application both as a cell carrier and wound dressing material. Swelling, hardness, adhesive force (wet), porosity, and hydrophilicity (dry) of the hydrogels were dependent on the degree of cross-linking and the amount of AA incorporated in the hydrogels. However, water vapor transmission rate, pore size, hydrophilicity (semidry), and topography were similar between all formulations, leading to a similar cell attachment and proliferation profile. At the cellular level, the hydrogel demonstrated rapid cell adhesion, maintained HDFs viability and morphology, restricted cellular migration, and facilitated fast transfer of cells. At the molecular level, the hydrogel affected nine wound-healing genes (IL6, IL10, MMP2, CTSK, FGF7, GM-CSF, TGFB1, COX2, and F3). The findings indicate that the BNC/AA hydrogel is a potential biomaterial that can be employed as a wound-dressing material to incorporate HDFs for the acceleration of wound healing.

Original languageEnglish
Pages (from-to)39532-39543
Number of pages12
JournalACS Applied Materials and Interfaces
Volume10
Issue number46
DOIs
Publication statusPublished - 21 Nov 2018

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Tissue regeneration
Molecular interactions
Hydrogel
Fibroblasts
Hydrogels
Biocompatible Materials
Composite materials
Hydrophilicity
Biomaterials
Cell adhesion
Steam
Granulocyte-Macrophage Colony-Stimulating Factor
Acrylics
Interleukin-10
Topography
Dosimetry
Pore size
Swelling
Electron beams
Interleukin-6

Keywords

  • cell carrier
  • cell-material interaction
  • scaffold
  • skin wound healing
  • wound dressing

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

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title = "Cellular and Molecular Interaction of Human Dermal Fibroblasts with Bacterial Nanocellulose Composite Hydrogel for Tissue Regeneration",
abstract = "The evaluation of the interaction of cells with biomaterials is fundamental to establish the suitability of the biomaterial for a specific application. In this study, the properties of bacterial nanocellulose/acrylic acid (BNC/AA) hydrogels fabricated with varying BNC to AA ratios and electron-beam irradiation doses were determined. The manner these hydrogel properties influence the behavior of human dermal fibroblasts (HDFs) at the cellular and molecular levels was also investigated, relating it to its application both as a cell carrier and wound dressing material. Swelling, hardness, adhesive force (wet), porosity, and hydrophilicity (dry) of the hydrogels were dependent on the degree of cross-linking and the amount of AA incorporated in the hydrogels. However, water vapor transmission rate, pore size, hydrophilicity (semidry), and topography were similar between all formulations, leading to a similar cell attachment and proliferation profile. At the cellular level, the hydrogel demonstrated rapid cell adhesion, maintained HDFs viability and morphology, restricted cellular migration, and facilitated fast transfer of cells. At the molecular level, the hydrogel affected nine wound-healing genes (IL6, IL10, MMP2, CTSK, FGF7, GM-CSF, TGFB1, COX2, and F3). The findings indicate that the BNC/AA hydrogel is a potential biomaterial that can be employed as a wound-dressing material to incorporate HDFs for the acceleration of wound healing.",
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AU - Ng, Pei Yuen

AU - Ng, Shiow Fern

AU - Ariffin, Hidayah

AU - Mohd Amin, Mohd Cairul Iqbal

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