Cell death induced by hydroxyapatite on L929 fibroblast cells.

S. H. Inayat-Hussain, N. F. Rajab, H. Roslie, A. A. Hussin, A. M. Ali, B. O. Annuar

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Biomaterials intended for end-use application as bone-graft substitutes have to undergo safety evaluation. In this study, we investigated the in vitro cytotoxic effects especially to determine the mode of death of two hydroxyapatite compounds (HA2, HA3) which were synthesized locally. The methods used for cytotoxicity was the standard MTT assay whereas AO/PI staining was performed to determine the mode of cell death in HA treated L929 fibroblasts. Our results demonstrated that both HA2 and HA3 were not significantly cytotoxic as more than 75% cells after 72 hours treatment were viable. Furthermore, we found that the major mode of cell death in HA treated cells was apoptosis. In conclusion, our results demonstrated that these hydroxyapatite compounds are not cytotoxic where the mode of death was primarily via apoptosis.

Original languageEnglish
Pages (from-to)176-177
Number of pages2
JournalThe Medical journal of Malaysia
Volume59 Suppl B
Publication statusPublished - May 2004

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Durapatite
Cell Death
Fibroblasts
Apoptosis
Bone Substitutes
Biocompatible Materials
Staining and Labeling
Transplants
Safety
In Vitro Techniques

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Inayat-Hussain, S. H., Rajab, N. F., Roslie, H., Hussin, A. A., Ali, A. M., & Annuar, B. O. (2004). Cell death induced by hydroxyapatite on L929 fibroblast cells. The Medical journal of Malaysia, 59 Suppl B, 176-177.

Cell death induced by hydroxyapatite on L929 fibroblast cells. / Inayat-Hussain, S. H.; Rajab, N. F.; Roslie, H.; Hussin, A. A.; Ali, A. M.; Annuar, B. O.

In: The Medical journal of Malaysia, Vol. 59 Suppl B, 05.2004, p. 176-177.

Research output: Contribution to journalArticle

Inayat-Hussain, SH, Rajab, NF, Roslie, H, Hussin, AA, Ali, AM & Annuar, BO 2004, 'Cell death induced by hydroxyapatite on L929 fibroblast cells.', The Medical journal of Malaysia, vol. 59 Suppl B, pp. 176-177.
Inayat-Hussain SH, Rajab NF, Roslie H, Hussin AA, Ali AM, Annuar BO. Cell death induced by hydroxyapatite on L929 fibroblast cells. The Medical journal of Malaysia. 2004 May;59 Suppl B:176-177.
Inayat-Hussain, S. H. ; Rajab, N. F. ; Roslie, H. ; Hussin, A. A. ; Ali, A. M. ; Annuar, B. O. / Cell death induced by hydroxyapatite on L929 fibroblast cells. In: The Medical journal of Malaysia. 2004 ; Vol. 59 Suppl B. pp. 176-177.
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