Cell-cell contact configurations by dielectrophoresis for electrofusion

Study on directions, stability and dielectric heating effect

Mohd Anuar Md Ali, Burhanuddin Yeop Majlis, Zahhar Nisa Azman, Aminuddin A. Kayani

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Electrofusion can be performed by forming cell-cell contact, while simultaneously applying pulsed high voltage electric fields to induce the fusion. Mechanisms of forming cell-cell contact has been demonstrated by various methods including biochemical, chemical, mechanical and electrical methods. Dielectrophoresis has been utilized in electrical based cell-cell contact formation. In this work, we investigated the potential configurations of cell-cell contact by dielectrophoresis including cells cluster and cells chain by microfluidic dielectrophoresis platforms using microtip based electrodes. We simulated the positive-and negative-dielectrophoretic response of yeast cells in deionized water, the distribution of electric fields and the dielectric heating effect on suspending medium temperature. We performed experimental work to investigate the cell-cell contact availability, directions, stability and dielectric heating effect under these configurations.

Original languageEnglish
Title of host publicationIECBES 2016 - IEEE-EMBS Conference on Biomedical Engineering and Sciences
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages539-544
Number of pages6
ISBN (Electronic)9781467377911
DOIs
Publication statusPublished - 3 Feb 2017
Event2016 IEEE-EMBS Conference on Biomedical Engineering and Sciences, IECBES 2016 - Kuala Lumpur, Malaysia
Duration: 4 Dec 20168 Dec 2016

Other

Other2016 IEEE-EMBS Conference on Biomedical Engineering and Sciences, IECBES 2016
CountryMalaysia
CityKuala Lumpur
Period4/12/168/12/16

Fingerprint

Dielectric heating
Electrophoresis
heating
configurations
cells
Electric fields
Deionized water
Microfluidics
Yeast
Fusion reactions
Cells
Availability
Electrodes
Electric potential
Temperature
electric fields
yeast

Keywords

  • cell chain
  • cell cluster
  • cell-cell contact
  • dielectrophoresis
  • electrofusion
  • yeast

ASJC Scopus subject areas

  • Biomedical Engineering
  • Instrumentation

Cite this

Ali, M. A. M., Yeop Majlis, B., Azman, Z. N., & Kayani, A. A. (2017). Cell-cell contact configurations by dielectrophoresis for electrofusion: Study on directions, stability and dielectric heating effect. In IECBES 2016 - IEEE-EMBS Conference on Biomedical Engineering and Sciences (pp. 539-544). [7843508] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/IECBES.2016.7843508

Cell-cell contact configurations by dielectrophoresis for electrofusion : Study on directions, stability and dielectric heating effect. / Ali, Mohd Anuar Md; Yeop Majlis, Burhanuddin; Azman, Zahhar Nisa; Kayani, Aminuddin A.

IECBES 2016 - IEEE-EMBS Conference on Biomedical Engineering and Sciences. Institute of Electrical and Electronics Engineers Inc., 2017. p. 539-544 7843508.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Ali, MAM, Yeop Majlis, B, Azman, ZN & Kayani, AA 2017, Cell-cell contact configurations by dielectrophoresis for electrofusion: Study on directions, stability and dielectric heating effect. in IECBES 2016 - IEEE-EMBS Conference on Biomedical Engineering and Sciences., 7843508, Institute of Electrical and Electronics Engineers Inc., pp. 539-544, 2016 IEEE-EMBS Conference on Biomedical Engineering and Sciences, IECBES 2016, Kuala Lumpur, Malaysia, 4/12/16. https://doi.org/10.1109/IECBES.2016.7843508
Ali MAM, Yeop Majlis B, Azman ZN, Kayani AA. Cell-cell contact configurations by dielectrophoresis for electrofusion: Study on directions, stability and dielectric heating effect. In IECBES 2016 - IEEE-EMBS Conference on Biomedical Engineering and Sciences. Institute of Electrical and Electronics Engineers Inc. 2017. p. 539-544. 7843508 https://doi.org/10.1109/IECBES.2016.7843508
Ali, Mohd Anuar Md ; Yeop Majlis, Burhanuddin ; Azman, Zahhar Nisa ; Kayani, Aminuddin A. / Cell-cell contact configurations by dielectrophoresis for electrofusion : Study on directions, stability and dielectric heating effect. IECBES 2016 - IEEE-EMBS Conference on Biomedical Engineering and Sciences. Institute of Electrical and Electronics Engineers Inc., 2017. pp. 539-544
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