Abstract
Electrofusion can be performed by forming cell-cell contact, while simultaneously applying pulsed high voltage electric fields to induce the fusion. Mechanisms of forming cell-cell contact has been demonstrated by various methods including biochemical, chemical, mechanical and electrical methods. Dielectrophoresis has been utilized in electrical based cell-cell contact formation. In this work, we investigated the potential configurations of cell-cell contact by dielectrophoresis including cells cluster and cells chain by microfluidic dielectrophoresis platforms using microtip based electrodes. We simulated the positive-and negative-dielectrophoretic response of yeast cells in deionized water, the distribution of electric fields and the dielectric heating effect on suspending medium temperature. We performed experimental work to investigate the cell-cell contact availability, directions, stability and dielectric heating effect under these configurations.
| Original language | English |
|---|---|
| Title of host publication | IECBES 2016 - IEEE-EMBS Conference on Biomedical Engineering and Sciences |
| Publisher | Institute of Electrical and Electronics Engineers Inc. |
| Pages | 539-544 |
| Number of pages | 6 |
| ISBN (Electronic) | 9781467377911 |
| DOIs | |
| Publication status | Published - 3 Feb 2017 |
| Event | 2016 IEEE-EMBS Conference on Biomedical Engineering and Sciences, IECBES 2016 - Kuala Lumpur, Malaysia Duration: 4 Dec 2016 → 8 Dec 2016 |
Other
| Other | 2016 IEEE-EMBS Conference on Biomedical Engineering and Sciences, IECBES 2016 |
|---|---|
| Country | Malaysia |
| City | Kuala Lumpur |
| Period | 4/12/16 → 8/12/16 |
Fingerprint
Keywords
- cell chain
- cell cluster
- cell-cell contact
- dielectrophoresis
- electrofusion
- yeast
ASJC Scopus subject areas
- Biomedical Engineering
- Instrumentation
Cite this
Cell-cell contact configurations by dielectrophoresis for electrofusion : Study on directions, stability and dielectric heating effect. / Ali, Mohd Anuar Md; Yeop Majlis, Burhanuddin; Azman, Zahhar Nisa; Kayani, Aminuddin A.
IECBES 2016 - IEEE-EMBS Conference on Biomedical Engineering and Sciences. Institute of Electrical and Electronics Engineers Inc., 2017. p. 539-544 7843508.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Cell-cell contact configurations by dielectrophoresis for electrofusion
T2 - Study on directions, stability and dielectric heating effect
AU - Ali, Mohd Anuar Md
AU - Yeop Majlis, Burhanuddin
AU - Azman, Zahhar Nisa
AU - Kayani, Aminuddin A.
PY - 2017/2/3
Y1 - 2017/2/3
N2 - Electrofusion can be performed by forming cell-cell contact, while simultaneously applying pulsed high voltage electric fields to induce the fusion. Mechanisms of forming cell-cell contact has been demonstrated by various methods including biochemical, chemical, mechanical and electrical methods. Dielectrophoresis has been utilized in electrical based cell-cell contact formation. In this work, we investigated the potential configurations of cell-cell contact by dielectrophoresis including cells cluster and cells chain by microfluidic dielectrophoresis platforms using microtip based electrodes. We simulated the positive-and negative-dielectrophoretic response of yeast cells in deionized water, the distribution of electric fields and the dielectric heating effect on suspending medium temperature. We performed experimental work to investigate the cell-cell contact availability, directions, stability and dielectric heating effect under these configurations.
AB - Electrofusion can be performed by forming cell-cell contact, while simultaneously applying pulsed high voltage electric fields to induce the fusion. Mechanisms of forming cell-cell contact has been demonstrated by various methods including biochemical, chemical, mechanical and electrical methods. Dielectrophoresis has been utilized in electrical based cell-cell contact formation. In this work, we investigated the potential configurations of cell-cell contact by dielectrophoresis including cells cluster and cells chain by microfluidic dielectrophoresis platforms using microtip based electrodes. We simulated the positive-and negative-dielectrophoretic response of yeast cells in deionized water, the distribution of electric fields and the dielectric heating effect on suspending medium temperature. We performed experimental work to investigate the cell-cell contact availability, directions, stability and dielectric heating effect under these configurations.
KW - cell chain
KW - cell cluster
KW - cell-cell contact
KW - dielectrophoresis
KW - electrofusion
KW - yeast
UR - http://www.scopus.com/inward/record.url?scp=85015639806&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85015639806&partnerID=8YFLogxK
U2 - 10.1109/IECBES.2016.7843508
DO - 10.1109/IECBES.2016.7843508
M3 - Conference contribution
AN - SCOPUS:85015639806
SP - 539
EP - 544
BT - IECBES 2016 - IEEE-EMBS Conference on Biomedical Engineering and Sciences
PB - Institute of Electrical and Electronics Engineers Inc.
ER -