Cell based therapy for osteoarthritis in a sheep model: gross and histological assessment.

H. Alfaqeh, Nor Hamdan Mohamad Yahaya, Chua Kien Hui, H. C. Chen, B. S. Aminuddin, Ruszymah Idrus

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Abstract

This study was to determine if autologous bone marrow mesenchymal stem cells (BMSCs) cultured in chondrogenic medium could repair surgically induced osteoarthritis. Sheep BMSCs were cultured in medium containing 5ng/ml TGFbeta3 + 50ng/ml IGF-1 for three weeks. The cultured cells were then suspended at density of 2x10(6) cell/ml and injected intraarticularly into the osteoarthritic knee joint. After six weeks, the distal head of the femur and the proximal tibial plateau were removed and stained with H&E. The results indicated that knee joints treated with autologous BMSCs cultured in chondrogenic medium showed clear evidence of articular cartilage regeneration in comparison with other groups.

Original languageEnglish
Pages (from-to)37-38
Number of pages2
JournalThe Medical journal of Malaysia
Volume63 Suppl A
Publication statusPublished - Jul 2008

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Cell- and Tissue-Based Therapy
Mesenchymal Stromal Cells
Osteoarthritis
Sheep
Bone Marrow
Knee Joint
Femur Head
Articular Cartilage
Insulin-Like Growth Factor I
Regeneration
Cultured Cells

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Cell based therapy for osteoarthritis in a sheep model : gross and histological assessment. / Alfaqeh, H.; Mohamad Yahaya, Nor Hamdan; Kien Hui, Chua; Chen, H. C.; Aminuddin, B. S.; Idrus, Ruszymah.

In: The Medical journal of Malaysia, Vol. 63 Suppl A, 07.2008, p. 37-38.

Research output: Contribution to journalArticle

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