cDNA cloning and expression analysis of the chalcone synthase gene (CHS) from Polygonum minus

Nur Diyana Roslan, Cheng Seng Tan, Ismanizan Ismaill, Zamri Zainal

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Flavonoids are secondary metabolites that are present at high levels in the Polugonum minus. In recent years, flavonoids have attracted the interest of researchers because of their antioxidant properties .Flavonoids are synthesised via the condensation of 4 coumaryl-CoA and 3 malonyl-CoA by the action of Chalcone synthase. A cDNA encoding a chalcone synthase was isolated from the leaves of Polygonum minus by rapid amplification of cDNA ends (RACE) and designated pmCHS (GenBank accession no. JQ801338). The full-length cDNA of P. minus pmCHS was 1472 bp with a 1179 bp open reading frame (ORF) that corresponded to a predicted protein of 392 amino acid deduced protein. In silico analysis showed that the calculated molecular weight and theoretical isoelectric point (pI) of pmCHS were 43.1 kDa and 5.78, respectively. Several important motifs, such as the product binding site, active site and dimer interface, were also successfully identified from the deduced amino acid sequence. Multiple sequence alignment indicated that the pmCHS sequence was highly conserved and shared high sequence identity (>90%) with chalcone synthases from other plants. Gene expression analysis via qRT-PCR showed that pmCHS was most highly expressed in the roots, showing a 10-fold increase compared to leaves and a 15-fold increase compared to stems . The specific mechanism underlying the high expression of pmCHS in the roots requires further investigation.

Original languageEnglish
Pages (from-to)777-783
Number of pages7
JournalAustralian Journal of Crop Science
Volume7
Issue number6
Publication statusPublished - May 2013

Fingerprint

naringenin-chalcone synthase
molecular cloning
flavonoids
malonyl coenzyme A
rapid amplification of cDNA ends
genes
sequence alignment
isoelectric point
active sites
secondary metabolites
open reading frames
leaves
binding sites
amino acid sequences
proteins
researchers
molecular weight
antioxidants
gene expression
amino acids

Keywords

  • CDNA cloning
  • Chalcone synthase
  • Flavonoid
  • In silico
  • Polygonum minus

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

cDNA cloning and expression analysis of the chalcone synthase gene (CHS) from Polygonum minus. / Roslan, Nur Diyana; Tan, Cheng Seng; Ismaill, Ismanizan; Zainal, Zamri.

In: Australian Journal of Crop Science, Vol. 7, No. 6, 05.2013, p. 777-783.

Research output: Contribution to journalArticle

Roslan, Nur Diyana ; Tan, Cheng Seng ; Ismaill, Ismanizan ; Zainal, Zamri. / cDNA cloning and expression analysis of the chalcone synthase gene (CHS) from Polygonum minus. In: Australian Journal of Crop Science. 2013 ; Vol. 7, No. 6. pp. 777-783.
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